Diabetes mellitus of graded severity was produced by withdrawing insulin from alloxan diabetic rats previously maintained on Protamine Zinc Insulin. The tissue distribution of H-3 palmitate-labeled chyle in the diabetic animals and in normal animals was compared fifteen minutes after its intravenous administration. Although hyperglycemia was apparent forty-eight hours after insulin withdrawal, it was only at seventy-two and ninety-six hours after insulin withdrawal that marked retention of radioactivity was apparent in blood and that adipose tissue, carcass and hepatic uptake of fatty acid was lower than normal. Lipoprotein lipase content of epididymal fat pads was significantly higher than normal in insulin-treated and lower than normal in the insulin-deprived rats. The findings indicate that insulin withdrawal in the alloxan diabetic rat results in delayed clearing of intravenously administered chylomicrons from blood. Initially this may be related in part to the defective uptake by adipose tissue which becomes depleted of lipoprotein lipase. More prolonged insulin deprivation, however, leads to a greatly expanded serum triglyceride pool which in itself could account for the markedly delayed clearing of chylomicrons in severe diabetes. DIABETES 16: 90-95, February, 1967. There are conflicting reports in the literature on the relationship between the clearing of dietary triglycerides and metabolism of carbohydrate. Fredrickson et al. 1 have claimed that carbohydrate feeding has no effect on the removal of intravenously administered chyle from the blood of dogs. Bierman and Hamlin 2 have claimed that insulin deprivation does not influence the rate of removal of C-14 labeled triglycerides from the blood of insulin-dependent human diabetics. On the other hand, Albrink and Man 3 have indicated that the degree of alimentary lipemia after a fat meal is less when the same amount of fat is fed with glucose and, more recently, Krut and Barsky 4 have claimed that From the

SUMMARYThe prevalence of five types of hyperlipoproteinemia identified by Fredrickson, Levy

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