No abstract

We have characterized the human gene NDUFA5 encoding a 13-kDa subunit of the mitochondrial respiratory chain complex I (NADH: ubiquinone oxidoreductase). The gene contains 5 exons and 4 introns, and spans 14 kb of genomic DNA. In the untranscribed region we observed potential transcription factor binding sites. We determined a single nucleotide variant (C/T) at –318, and its frequency in the German population. The functional gene was localised by FISH to 7q31 and by radiation hybrid panel near marker D7S648 in YAC 883_a_2.

Poster abstracts 62 nucleic acids, such as DNA, RNA and PNA, proteins, such as antibodies, peptides and lectins, as well as other classes of biomolecules such as carbohydrates and lipids. The '2 x 96' XNA format, as well as the '2 x 384' format under development, provide a flexible strategy for constructing custom 'user-defined' arrays. Use of the microscope slide footprint and the standard well spacing simplifies processing by allowing the use of a wide range of commercially available manual and robotic, handling and deposition equipment for slides and microtiter plates. XNA is compatible with most detection schemes including radiometric, fluorescence and chemiluminescence. Model hybridisation and immunoassay data will be presented.XNA was originally developed as a tool for studying biological self-assembly processes. Biology uses nanometer sized units, that is to say proteins, carbohydrates, lipids, etc. to build everything from viruses to elephants. In this context, developmental biology defines the frontier in biosciences. If we are to understand the molecular basis of developmental processes, then we must learn to fabricate coatings capable of mimicking biological surfaces in relevant dimensions, i.e. nanometers, and in relevant environments, i.e. aqueous, as tools for studying these (self-) assembly processes. XNA technology provides a tool for 'reverse engineering' self-assembly processes that will serve as the basis for the development of a new generation of diagnostic bioassays. Meltzer, Paul