A human cDNA encoding the homologue of NADH:ubiquinone oxidoreductase subunit B13

Genomic organization of the human complex I 13-kDa subunit gene NDUFA5

“…1B). The genomic DNA exon sequences are identical to that of the NDUFA5 cDNA obtained earlier by Pata et al (1997), however the NDUFA5 cDNA sequence as reported by Russell et al (1997) showed differences to ours. The first 9 nucleotides of the sequence published by Russell et al (1997) were not found in genomic DNA, and presumably result from a cloning artifact or genomic heterogeneity.…”

“…Multiple NDUFA5-related sequences are present in the human genome which include one copy of a functional gene (on chromosome 7) and several processed, intronless pseudogenes (Pata et al, 1997). The pseudogenes have been inserted into genomic DNA at distinct evolutionary timepoints, as indicated by their different degree of identity to NDUFA5 (data not shown).…”

“…Following the isolation of the human NDUFA5 cDNA (also referred to as B13) (Pata et al, 1997), we identified the human intron-containing NDUFA5 gene and localized it to chromosome 7 using PCR and human-hamster somatic cell hybrids. Filters containing an arrayed chromosome 7-specific cosmid library (Lehrach et al, 1990) were screened with a NDUFA5 cDNA probe.…”

Cloning of the human cDNA sequence encoding the NADH: Ubiquinone oxidoreductase MLRQ subunit

“…In the case of B 13 subunit protein, a high degree of conservation in amino acid sequence was observed in various species including plants and fungi (7). The nucleotide and amino acid sequences of MLRQ subunits also showed high homologies among human, bovine, and mouse (Fig.…”

“…Two nuclear encoded subunits of 51 kDa and 24 kDa have been characterized at nucleotide sequence level and their genes mapped (4,5,6). Recently, the sequence of another subunit (B13) has been reported (7). Here, we report the cloning and sequencing of a cDNA clone encoding human MLRQ subunit from fetal liver.…”