The indicators of the activity of lipid peroxidation and the antioxidant system were studied in dynamics in 158 patients with infectious mononucleosis depending on the severity of the clinical course of the disease. It is proved that lipid peroxidation is significantly activated in patients with infectious mononucleosis as the severity of the disease increases and therefore increases the oxidative activity of blood plasma, the concentration of dyne conjugates and malondialdehyde in the blood, decreases the activity of antioxidant enzymes of erythrocytes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase), glutathione peroxidase and glutathione reductase activity in plasma and lowering the concentration of total and reduced glutathione in the blood, as well as reduce the antioxidant activity of blood plasma and erythrocytes. Lipid peroxidation significantly increase and the activity of the antioxidant system decrease in patients with infectious mononucleosis in the acute period of the disease, as evidenced by the increased concentration of dyne conjugates, malonic dialdehyde and total oxidative plasma activity in the blood, reduced activity of the total antioxidant activity of plasma and erythrocytes, the decrease in the activity of antioxidant enzymes of erythrocytes catalase, superoxide dismutase, glutathione peroxidase, glutathione peroxidase and glutathione reductase of blood plasma, the decrease in the concentration of glutathione in the blood. The most pronounced disorders of lipid peroxidation and antioxidant system activity were found in patients with moderate-severe and severe infectious mononucleosis. The development of cytolytic syndrome in infectious mononucleosis is associated with the action of reactive oxygen forms species and lipid hydroperoxide.

Objective. The article is devoted to the study of the content of the main immune parameters in patients with infectious mononucleosis (IM) in the dynamics of the disease. Materials and methods. A clinical examination of IM patients (n = 60) and patients of the control group (n = 20) included the study of complaints, epidemiological history, history of disease and life, objective examination, standard instrumental and laboratory studies in dynamics, detection of EBV DNA in saliva and blood serum, and a comprehensive analysis of immune parameters. The main subpopulations of peripheral blood lymphocytes (CD3 +; CD4 +; CD8 +; CD16 +; CD8 + CD28 +; CD8 + CD28-; CD20 +; CD25 +) were determined by flow laser cytometry on a FACS-Calibur apparatus (USA) using monoclonal antibodies. For identification of INF-γ (Th1-cells), IL-4 (Th2-cells) in the cytoplasm of T-lymphocytes, monoclonal antibodies INF-γ-PC-5, IL-4-PE (eBioscience, Beckman Caulter, R & D System) were used. Results. A comprehensive study of the state of the subpopulations of reacting immune cells revealed significant violations of cellular parts of the immune response compared to the control group. It was established that the immune response in patients with IM during the height of the disease is characterized by an imbalance in the cell link (as evidenced by an increase in the content of CD3+, CD4+, and a simultaneous increase in the content of CD16+, CD25+). In the period of convalescence, violations have been identified that will persist without reaching the levels of the control group in a larger number of IM patients. Conclusion. The results obtained indicate significant changes in the structural characteristics of the cellular immunity system and the multidirectional immune response in IM. The progressive character of changes in immune parameters in IM indicates the formation of a secondary cellular immune imbalance, a change in the balance of immunoregulatory mediators towards the Th2 link during the formation of protracted and chronic forms of EBV-infection.

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Abstract. The study of the role of Epstein-Barr viral infection in the occurrence of various pathological conditions in humans is of great importance. This is due to the significant epidemiological role, since upon reaching the age of majority, more than 90% of people are infected of Epstein-Barr virus. The steady increase in the number of diseases caused by Epstein-Barr virus both among adults and children, which is associated with its specific tropism for immunocompetent cells, lifelong persistence and latent course necessitates a comprehensive study and development of effective treatment methods. Purpose of the work: to assess clinical and immunological parameters in order to identify a protracted course and predict unfavorable outcomes in patients with infectious mononucleosis caused by the Epstein-Barr virus. Materials and methods: To achieve this purpose, 60 patients with infectious mononucleosis were examined, among them 38 women (63.3%), 22 men (36.7%). The average age of the patients was 24.3±4.3 years. The material for the study was the patient's serum obtained in the dynamics of the disease. The complex of examination of patients with Infectious mononucleosis included clinical and biochemical methods, enzyme-linked immunosorbent assay, polymerase chain reaction method, immunogram. The research results were processed by the method of variation and correlation statistics using the Statistica 10.0 for Windows program. Results. The analysis of the obtained results made it possible to establish that in patients with infectious mononucleosis, changes were revealed in the system of cellular and humoral immunity and its multidirectionality. The progressive nature of changes in immune parameters indicates the formation of a secondary cellular immune imbalance, activation of the humoral link, a change in the balance of immunoregulatory mediators towards Th2 cells. In the acute period, statistically significant violations of the cellular link of immunity were established, which were characterized by an increase in the number of cells with killer activity: mature T-lymphocytes (CD3+), cytotoxic T-suppressor cells (CD8+), cells expressing the activation marker CD25+ (IL-2 receptor ) and increased Th1/Th2. Conclusions. Thus, changes in the cellular and humoral links of immunity in a favorable course of myocardial infarction are characterized by activation of the cellular and humoral links of immunity, which is manifested by an increase in the content of lymphocytes in the peripheral blood with the [CD3+; CD4+; CD8+; CD16+; CD20+], Th1 cells, IgA and IgM; (p<0.05) with a tendency to normalization during the period of convalescence. With the formation of chronic forms of Epstein-Barr viral infection, a progressive nature of changes in immune parameters is noted, which indicates the formation of a secondary lymphocytic cellular imbalance, activation of the humoral link, a change in the balance of immunoregulatory mediators towards Th2 (a decrease in the content of [CD3+; CD4+; CD8+; CD16+]; an increase in the content of CD20+; IgG levels; Th2 (IL-4+) Th1/Th2 (p<0.05) due to an increase in the relative content of Th2 cells.

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