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This study investigates the performance of a passive time-reversal mirror (TRM) combined with acoustic ray theory in localizing underwater sound sources with high frequencies (3–7 kHz). The TRM was installed on a floating buoy and comprised four hydrophones. The ray-tracing code BELLHOP was used to determine the transfer function between a sound source and a field point. The transfer function in the frequency domain obtained from BELLHOP was transformed into the time domain. The pressure field was then obtained by taking the convolution of the transfer function in the time domain with the time-reversed signals that were received by the hydrophones in the TRM. The location with the maximum pressure value was designated as the location of the source. The performance of the proposed methodology for source localization was tested in a towing tank and in the ocean. The aforementioned tests revealed that even when the distances between a source and the TRM were up to 1600 m, the distance deviations between estimated and actual source locations were mostly less than 2 m. Errors originated mainly from inaccurate depth estimation, and the literature indicates that they can be reduced by increasing the number of TRM elements and their apertures.

Staphylococcus aureus (S. aureus) forms biofilm that causes periprosthetic joint infections (PJIs) and osteomyelitis (OM) which are the intractable health problems in clinics. The silver-containing nanoparticles (AgNPs) are antibacterial nanomaterials with less cytotoxicity than the classic Ag compounds. Likewise, gold nanoparticles (AuNPs) have also been demonstrated as excellent nanomaterials for medical applications. Previous studies have showed that both AgNPs and AuNPs have anti-microbial or anti-inflammatory properties. We’ve developed a novel green chemistry that could generate the AuAg nanocomposites, through the reduction of tannic acid (TNA). The bioactivity of the nanocomposites was investigated in S. aureus biofilm-exposed human osteoblast cells (hFOB1.19). The current synthesis method is a simple, low-cost, eco-friendly, and green chemistry approach. Our results showed that the AuAg nanocomposites were biocompatible with low cell toxicity, and did not induce cell apoptosis nor necrosis in hFOB1.19 cells. Moreover, AuAg nanocomposites could effectively inhibited the accumulation of reactive oxygen species (ROS) in mitochondria and in rest of cellular compartments after exposing to bacterial biofilm (by reducing 0.78, 0.77-fold in the cell and mitochondria, respectively). AuAg nanocomposites also suppressed ROS-triggered inflammatory protein expression via MAPKs and Akt pathways. The current data suggest that AuAg nanocomposites have the potential to be a good therapeutic agent in treating inflammation in bacteria-infected bone diseases.

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