Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of alleles ranged from 3 to 8. The expected (H E ) and observed (H O ) heterozygosities were 0.37-0.78 and 0.05-0.52, respectively. All microsatellite loci are significantly deviated from Hardy-Weinberg expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its closely related species, M. floridulus.

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Ludwigia, a genus with rampant interspecific hybridization, is an ideal model for examining the impact of gene introgression and polyploidization on species diversification. We examined the evolution of the Ludwigia sect. Isnardia, which is a polyploid complex (x = 8) of five species distributed in North America. The phylogeny of L. sect. Isnardia was reconstructed based on sequences of cpDNA atpB­rbcL intergenic spacer and nrITS (internal transcribed spacer). Most L. sect. Isnardia species are polyphyletic at both loci, indicating possible recurrent hybridization, based on maximum likelihood trees rooted at L. sect. Microcarpium. A minimum spanning network was also constructed to examine genealogical relationships among haplotypes. In the cpDNA network, haplotypes of tetraploid L. spathulata are nested at the most interior nodes, suggesting a parental genome origin of an extinct diploid or an unsampled extant diploid with a DD cytotype. Allopolyploid L. arcuata contributed its cpDNA to hexaploid L. repens and L. brevipes. ITS haplotypes of diploid L. palustris were clustered with L. spathulata, suggesting L. palustris as the paternal genome donor. Ludwigia brevipes may have arisen via bi3directional hybridization between L. palustris and L. arcuata followed by polyploidy. Genetic and phylogenetic analyses using molecular markers with different inheritance modes provided sufficient insights into the reticulate evolution of the species of L. sect. Isnardia. Interspecific hybridization and polyploidy play a key role in the species evolution in L. sect. Isnardia.

The presence of antibiotic residues in seafood and their effect on public health constitute a matter of concern for consumers worldwide. Antibiotic residues can have adverse effects on both humans and animals, especially residues of banned veterinary drugs. In this study, we applied a validated method to analyze veterinary drug residues in shrimp, including the levels of banned chloramphenicol, malachite green, leucomalachite green, and four nitrofuran metabolites as well as thiamphenicol, florfenicol, and five quinolones, which have no recommended maximum residual levels in shrimp tissues in Taiwan. We collected 53 samples of whiteleg, grass, or giant river shrimp from Taiwanese aquafarms and production areas from July 2016 to December 2017. We found 0.31 ng/g of a chloramphenicol in one grass shrimp, 5.62 ng/g of enrofloxacin in one whiteleg shrimp, 1.52 ng/g of flumequine in one whiteleg shrimp, and 1.01 ng/g of flumequine in one giant river shrimp, indicating that 7.55% of the samples contained veterinary drug residues. We evaluated the health risk by deriving the estimated daily intake (EDI). The quinolone residue EDI was below 1.0% of the acceptable daily intake recommended by the United Nations Food and Agriculture Organization and World Health Organization. The risk was thus discovered to be negligible, indicating no immediate health risk associated with shrimp consumption. The present findings can serve as a reference regarding food safety and in monitoring of the veterinary drug residues present in aquatic organisms. Continual monitoring of residues in shrimp is critical for further assessment of possible effects on human health.

Liver disorders have been recognized as one major health concern. Fucoidan, a sulfated polysaccharide extracted from the brown seaweed Fucus serratus, has previously been reported as an anti-inflammatory and antioxidant. However, the discovery and validation of its hepatoprotective properties and elucidation of its mechanisms of action are still unknown. The objective of the current study was to investigate the effect and possible modes of action of a treatment of fucoidan against thioacetamide (TAA)-induced liver injury in male C57BL/6 mice by serum biochemical and histological analyses. The mouse model for liver damage was developed by the administration of TAA thrice a week for six weeks. The mice with TAA-induced liver injury were orally administered fucoidan once a day for 42 days. The treated mice showed significantly higher body weights; food intakes; hepatic antioxidative enzymes (catalase, glutathione peroxidase (GPx), and superoxide dismutase (SOD)); and a lower serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and C-reactive protein (CRP) levels. Additionally, a reduced hepatic IL-6 level and a decreased expression of inflammatory-related genes, such as cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) mRNA was observed. These results demonstrated that fucoidan had a hepatoprotective effect on liver injury through the suppression of the inflammatory responses and acting as an antioxidant. In addition, here, we validated the use of fucoidan against liver disorders with supporting molecular data.