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Aim:To study the major histocompatibility complex (MHC) Class II DRB1 gene polymorphism in Rohilkhandi goat using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and nucleotide sequencing techniques.Materials and Methods:DNA was isolated from 127 Rohilkhandi goats maintained at sheep and goat farm, Indian Veterinary Research Institute, Izatnagar, Bareilly. A 284 bp fragment of exon 2 of DRB1 gene was amplified and digested using BsaI and TaqI restriction enzymes. Population genetic parameters were calculated using Popgene v 1.32 and SAS 9.0. The genotypes were then sequenced using Sanger dideoxy chain termination method and were compared with related breeds/species using MEGA 6.0 and Megalign (DNASTAR) software.Results:TaqI locus showed three and BsaI locus showed two genotypes. Both the loci were found to be in Hardy–Weinberg equilibrium (HWE), however, population genetic parameters suggest that heterozygosity is still maintained in the population at both loci. Percent diversity and divergence matrix, as well as phylogenetic analysis revealed that the MHC Class II DRB1 gene of Rohilkhandi goats was found to be in close cluster with Garole and Scottish blackface sheep breeds as compared to other goat breeds included in the sequence comparison.Conclusion:The PCR-RFLP patterns showed population to be in HWE and absence of one genotype at one locus (BsaI), both the loci showed excess of one or the other homozygote genotype, however, effective number of alleles showed that allelic diversity is present in the population. Sequence comparison of DRB1 gene of Rohilkhandi goat with other sheep and goat breed assigned Rohilkhandi goat in divergence with Jamanupari and Angora goats.

The current dataset is generated via bio-computational approach by surveying of INGI/RIME and SLACS CRE transposable elements (TEs) in latest update of Trypanosoma brucei genome. The distribution dataset (Supplementary File 1) shows the chromosome wise distribution of INGI/RIME and SLACS CRE transposable elements with the status of their -5′ and -3′ ends, genomic coverage and further elemental description about the completeness on the element. The 5′ upstream flanking sequence of 100 bp was then analyzed to find out possible regions that could act as insertion hotspots. The Fig. 1 represents the ten different motifs found in the 5′ flanking region of the INGI/RIME and SLACS CRE elements. The Supplementary File 2 describes the distribution of these ten motifs in different locations in Trypanosoma brucei genome. These new locations where motifs were found may provide useful information to track the future transposition events of INGI/RIME and SLACS CRE elements in different Trypanosoma species.

Aim: To study the genome profile of Chital deer (A.axis) by microsatellite markers using faecal DNA & to assess its usefulness as non invasive method of genotyping. Materials and Methods:DNA was isolated from 44 animals from faeces and blood, out of which 26 (3 from blood and 23 from faeces) were subjected for genotyping. The PCR products of all the ten microsatellite loci were subjected to 6 % Urea-PAGE to differentiate allelic size.

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