Mastitis is one of the most considerable and costly diseases for dairy herds, and Staphylococcus spp. is known to be the main causative agent. Although antibiotics are widely used in the treatment of mastitis, this can cause both antibiotic residues in milk and the risk of antibiotic resistance occurrence in bacteria. Thus, in recent years, researchers have focused on alternative treatments for this disease and plants extracts are investigated for this purpose. Pomegranate is widely used as a dye, ornament, and medicinal plants in the industry, and the species has a particularly high economic value in Turkey. This study aims to investigate in vitro the antioxidant and antibacterial properties of the pomegranate flower’s extracts against different Staphylococcus species associated with bovine mastitis. To this aim, pomegranate flowers were collected from different regions in Turkey and extracts were prepared with three different solvents (methanol, ethanol, and water). The retention factor values of the ethanol extract were determined by thin-layer chromatography. The antibacterial activity tests were carried out via the disk diffusion method. In addition, the extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radicals for antioxidant activity. Four retention factors (0.79, 0.67, 0.58, and 0.33 points) were found for the ethanol extract. The methanol extract showed the highest inhibition zones against coagulase-negative Staphylococcus-37 (CNS-37) and S. aureus-18. The lowest MIC was 6500 μg/mL. The highest antioxidant activity was observed in methanol extracts. As a result, the extracts of pomegranate flowers showed a high antioxidant and antibacterial potential against the examined mastitis pathogens.

Food spoilage pathogens threaten to human health. In this work was studied biological activity of plant known as the “kurtludiken” in Turkey and it is contributed to the literature. Cardopatium corymbosum L. from Mugla region were collected for antimicrobial and antioxidant activity studies. The antimicrobial activity studies were done by disc diffusion method. A total of 8 microorganisms were used at this study, one of them is yeast and the others are bacteria. The 2,2′-azinobis-(3 ethyl benzothiazoline-6-sulfonic acid) (ABTS•+) was used for non-enzymatic antioxidant activity experiments. There are few studies on the biological activities of Cardopatium corymbosum. The aim of this study is to determine the antimicrobial and antioxidant effects of Cardopatium corymbosum against food pathogens. The radical scavenging percentage of plant was found to be 13%. As a result, Cardopatium corymbosum have antimicrobial and antioxidant capacities.

Up to day, very little work has been done on the antimicrobial activity of spices extracts against food pathogens. Additionally, there is very little information about the antioxidant activities of spices. The aim of this work was to investigate the antimicrobial effects of spices extracts against food pathogens, and its non-enzymatic antioxidant potentials. The plants have been provided from C2 region of Mugla and local herbalists. Antimicrobial activities of plants were evaluated using disc diffusion method. The extracts showed maximum inhibition zone against various microorganisms, and the zone was 8 mm. Candida albicans and Listeria monocytogenes showed the lowest sensitivity to different extracts (3250 µg/ml). In addition, the extracts were tested against the DPPH [2,2-Diphenyl-1-picrylhydrazyl] free-radical for antioxidant activity. A lot of extracts were displayed a high antioxidant activity. The highest antioxidant activity was determined on Nigella sativa (84%). In this study determined that the spices extracts have antimicrobial and antioxidant properties.

There is an expanding interest in medicinal and aromatic plants as a natural alternative to synthetic drugs, especially to antimicrobial agents due to the growing problem of antibiotic resistance. In recent years, a lot of reports have been published on the antimicrobial activity of the plant extracts. This study was used Lavandula angustifolia L., Mentha piperita L., and Ribes nigrum L. as plant materials. This study aims to test the plant extracts against oral bacteria. Its purpose is to produce directly comparable, quantitative, antimicrobial data, and in addition to containing very little information of the different extracts against oral pathogens. Disc diffusion method was studied for antimicrobial activity tests. Also, minimum inhibitory concentration (MIC) values were determined in this study. Additionally, the extracts were tested against stable 2,2-diphenyl-1-picryl-hydrazyl-hydrate• (DPPH•) free radicals for non-enzymatic antioxidant activity. This study was used Trolox (6-hydroxy-2,5,7,8-tetra-methyl chroman-2-carboxylic acid) as standard. The extracts showed different inhibition zones against bacteria. The methanol extract of Lavandula showed the highest inhibition zone against the oral pathogen MBKK5. The positive control was penicillin (10 μg). The lowest MIC value was taken at 6500 μg /ml concentration of the plant extracts. The highest DPPH• radical scavenging activity was found in Ribes nigrum extract as 36%. As a result, plant extracts have antibacterial and antioxidant potential.