We have examined the effect of dietary nutrient composition on the growth, body composition and blood chemistry of juvenile olive flounder (Paralichthys olivaceus) maintained on two different feeding regimes. Three experimental diets [HP (high protein), HE (high energy) and IPE (intermediate protein and energy)] were crossed in a two-factor design with the two feeding regimes, which consisted of 2WS + 2WF (2-week starvation and then 2-week feeding) and 4WS + 4WF (4-week starvation and then 4-week feeding)], for 8 weeks. The total days of feeding in all treatments were the same. The final weight of fish was affected by feeding regime, but not by dietary nutrient composition. Long-term starving and feeding (the 4WS + 4WF treatments) yielded higher growth than the alternating short-term starving and feeding regime (2WS + 2WF treatments). The feed consumption of fish was affected by dietary nutrient composition, but not by feeding regime, while the feed efficiency of fish was affected by feeding regime, but not by dietary nutrient composition. Moisture, crude protein and crude lipid contents of the liver of the fish were affected by dietary nutrient composition, but not by feeding regime.
A polarization-independent multilayer dielectric diffraction grating with a low aspect ratio and high diffraction efficiency was designed and fabricated. The diffraction grating designed with a grating density of 1200 lines/mm had an aspect ratio of 0.59, mean polarization-independent diffraction efficiency in the Littrow angle of ± 2.5 ∘ , and 1030–1080 nm wavelength range of 97.2%. The designed grating was fabricated using ion assisted deposition and reactive ion etching techniques. The mean polarization-independent diffraction efficiency of the fabricated grating was 96.1%, and its standard deviation was 0.68%. The fabricated diffraction grating was irradiated with a 1064 nm cw laser, with a power density of 30 k W / c m 2 , for 1 min to measure the temperature change before and after the laser application. It was verified that the temperature variation of the diffraction grating without heat treatment was 8.8°C, and the temperature variation after heat treatment at 400°C decreased to 2.3°C.
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