Abstract. BACKGROUND:A high incidence of root and crown rot of strawberries (Macrophomina phaseolina) has been observed in major strawberry production regions. In Chile, the pathogen was reported in strawberry plants in 2013. A strategy for disease management is the use of resistant cultivars. OBJECTIVE: The aim of this study was to evaluate the susceptibility of eleven strawberry cultivars to the disease. METHODS: Two trials were conducted. A first trial was performed under greenhouse conditions, with plants propagated by runners and inoculated by oat seeds infected with M. phaseolina. The impact of the disease on the growth of the plants and incidence were assessed. The second trial was performed in a growth chamber. Plants were obtained by in-vitro multiplication and were inoculated with a suspension of sclerotia of the same isolate. Plant mortality was assessed. RESULTS: Significant differences in susceptibility were observed among the eleven strawberry cultivars, with 'Florida Festival', 'Amiga' and 'Naiad' as the least susceptible cultivars, while 'Florida Fortuna' was the most susceptible cultivar in both trials. CONCLUSIONS: Differences were detected in terms of susceptibility of cultivars to root and crown rot of strawberry caused by M. phaseolina. This work contributes to knowledge about the susceptibility of strawberry cultivars to this disease.