The pan-cancer analysis of whole genomes The expansion of whole-genome sequencing studies from individual ICGC and TCGA working groups presented the opportunity to undertake a meta-analysis of genomic features across tumour types. To achieve this, the PCAWG Consortium was established. A Technical Working Group implemented the informatics analyses by aggregating the raw sequencing data from different working groups that studied individual tumour types, aligning the sequences to the human genome and delivering a set of high-quality somatic mutation calls for downstream analysis (Extended Data Fig. 1). Given the recent meta-analysis

In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.

The turbulent Rayleigh-Taylor instability is investigated in the limit of strong mode-coupling using a variety of high-resolution, multimode, three dimensional numerical simulations ͑NS͒. The perturbations are initialized with only short wavelength modes so that the self-similar evolution ͑i.e., bubble diameter D b ϰamplitude h b) occurs solely by the nonlinear coupling ͑merger͒ of saturated modes. After an initial transient, it is found that h b ϳ␣ b Agt 2 , where AϭAtwood number, gϭacceleration, and tϭtime. The NS yield D b ϳh b /3 in agreement with experiment but the simulation value ␣ b ϳ0.025Ϯ0.003 is smaller than the experimental value ␣ b ϳ0.057Ϯ0.008. By analyzing the dominant bubbles, it is found that the small value of ␣ b can be attributed to a density dilution due to fine-scale mixing in our NS without interface reconstruction ͑IR͒ or an equivalent entrainment in our NS with IR. This may be characteristic of the mode coupling limit studied here and the associated ␣ b may represent a lower bound that is insensitive to the initial amplitude. Larger values of ␣ b can be obtained in the presence of additional long wavelength perturbations and this may be more characteristic of experiments. Here, the simulation data are also analyzed in terms of bubble dynamics, energy balance and the density fluctuation spectra.

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High-resolution X-ray observations have revealed cavities and ''cold fronts'' with sharp edges in temperature and density within galaxy clusters. Their presence poses a puzzle, since these features are not expected to be hydrodynamically stable or to remain sharp in the presence of diffusion. However, a moving core or bubble in even a very weakly magnetized plasma necessarily sweeps up enough magnetic field to build up a dynamically important sheath; the layer's strength is set by a competition between ''plowing up'' and slipping around of field lines, and depends primarily on the ram pressure seen by the moving object. In this inherently three-dimensional problem, our analytic arguments and numerical experiments show that this layer modifies the dynamics of a plunging core, greatly modifying the hydrodynamic instabilities and mixing, changing the geometry of stripped material, and slowing the core through magnetic tension. We derive an expression for the maximum magnetic field strength and thickness of the layer, as well as for the opening angle of the magnetic wake. The morphology of the magnetic draping layer implies the suppression of thermal conduction across the layer, thus conserving strong temperature gradients. The intermittent amplification of the magnetic field as well as the injection of magnetohydrodynamic turbulence in the wake of the core is identified to be due to vorticity generation within the magnetic draping layer. These results have important consequences for understanding the complex gas-dynamical processes of the intracluster medium and apply quite generally to motions through other magnetized environments, e.g., the interstellar medium.