Purpose. To perform a quantitative and qualitative impact assessment from point and diffuse sources of anthropo genic pollution on the Prut River basin within Ukraine. Methodology. Based on the developed statistical information and the use of the Guidance Paper on Impact Anal ysis and Risk Assessment in accordance with the EU Water Directive (EU project "Environmental protection of in ternational river basins"), load calculations have been carried out on four factors influencing the Prut ecosystem, namely, volumes of discharges of untreated sewage, the total share of sewage in the river, from agriculture and live stock production. findings. The main anthropogenic loads on the Prut river basin within IvanoFrankivsk and Chernivtsi regions of Ukraine from point and diffuse sources have been analyzed and calculated. In fact, it has been confirmed that diffuse sources are the main pollutants of the Prut ecosystem. Noncanalized area caused the emission of organic and nutri ent compounds into the river basin. According to the calculation of the indicators of diffuse load, water massifs in Kitsman, Kolomyia and Snyatyn districts are "possibly at risk" from agriculture, and in Hertzayivsky district-from livestock. The largest point polluters in the Prut River are communal enterprises of the settlements in Kolomyia and Chernivtsi. originality. The methodology for estimating loads on the river basin has been further developed by calculating load indicators, which allows the development and implementation of effective methods and tools for controlling the ecological safety of aquatic ecosystems. Practical value. The results obtained are the basis for adapting European approaches to integrated water resources management and developing measures to minimize the risks from human activities in the Prut basin. The work has been implemented at the national level in the process of drafting the Prut River Basin Management Plan in the frame work of the international project "Environmental protection of international river basins" (EPIRB) and improvement of the water monitoring system in the Carpathian National Nature Park.

The article summarizes the results of studies of the effectiveness of photodynamic therapy using a new domestic photosensitizer liposomal borated chlorin e6 (LBC) after its parenteral administration (intraperitoneal and intravenous). Antitumor efficacy was evaluated in rats with M-1 sarcoma and PC-1 alveolar liver cancer and mice with B16 melanoma and Ehrlich’s carcinoma, which were transplanted subcutaneously into the thigh area of the animals. The aim of the study was to determine the optimal regimes of photodynamic therapy that would allow achieving the maximum antitumor effect up to 21 days after the photodynamic therapy. The therapy was carried out under the control of the accumulation of the photosensitizer in the tumor and surrounding tissues of the thigh by selecting the doses of the drug and the parameters of laser radiation (energy density and power density). The effectiveness of therapy was assessed by the inhibition of tumor growth, by the percentage of animals with complete tumor regression, by the absolute growth rate in animals with continued tumor growth compared to controls. The results of our studies have shown that the domestic photosensitizer liposomal borated chlorin e6 has high antitumor activity in vivo. In an experimental study of the photosensitizer under certain PDT modes, the maximum antitumor effect (complete tumor regression in 100% of animals) was obtained up to 21 days after PDT in all tumor models used.

Efficacy of photodynamic therapy with intraperitoneal introduction photosensitizer Amidoaminchlorine on rat sarcoma M1 model was studied. The aim of this study was to determine minimum effective dose of photosensitizer and parameters of laser radiation for achievement of tumor full regress. Dynamics of photosensitizer accumulation in tumor and healthy tissues was studied to definite the time of photodynamic therapy. Antitumor efficacy different doses of photosensitizer and various parameters of laser radiation were investigated. As a result, optimal term of photodynamic therapy, photosensitizer minimum effective dose and parameters of laser irradiation were established.

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