Concrete floors are most commonly used in animal housing. However, the specific environment of livestock buildings (moisture, urine, disinfectants) has a negative effect on concrete and leads to its corrosion. The influence of chemical and physical factors on concrete is reinforced by the development of microorganisms, which quickly adapt and use concrete as a living environment. To reduce the influence of an aggressive environment on the concrete floor, an experimental mixture of dry disinfectants was proposed. The components of the disinfection mixture have been selected taking into account the safety for animals and humans. The TPD-MS method was used to determine the change in the chemical composition of concrete. To study the microstructure of concrete, the method of scanning electron microscopy was used. Microbiological studies revealed bacteria A. Thiooxidans, S. aureus, E. coli, S. enteritidis, S. Сholeraesuis, C. Perfringen and micromycetes of the genus Cladosporium, Fusariums, Aspergillus, which contribute to the development of biological corrosion of concrete in livestock buildings. The fact of the negative impact of concentrated disinfectants on the structure of concrete was also established. As a result of the studies carried out, it was proved that a mixture of dry components for disinfection exhibits antimicrobial properties to varying degrees to the strains of field isolates of bacteria and fungi isolated in a pig-breeding farm. It was found that when using the proposed mixture of dry disinfectants in the research room of the pigsty, the relative humidity decreases by 38.5 %; ammonia content – by 46.2 %; hydrogen sulfide – by 57.8 %; microbial bodies – by 74.7 %, compared with the control room. It has been experimentally proven that the proposed mixture of dry disinfecting components has hygroscopic and antimicrobial properties and is promising for use in livestock farms.
In the present study, we observed in vitro the rate and stages of exogenous development of the nematode Trichuris sylvilagi Tiner, 1950 isolated from the European hare, Lepus europaeus. The viability of eggs and embryos at different temperatures was investigated. It was found that the most favorable temperature for embryonic development of the species was 30 °C, with the formation of 88.67 % of viable eggs with a motile larva in 20 days at laboratory conditions. With a decrease in temperature to 20 °C, the embryogenesis occurred in 32 days. The viability of eggs cultured at 20 °C decreased: 68.0 % of eggs reached larval stage, and 32.0 % of eggs died. At 10 °C eggs did not develop to infective stage. On the 32nd day of cultivation at 10 °C, 27.33 % of eggs remained at the zygote stage, 52.0 % at the stage of blastomere cleavage and formation, and 20.67 % died. Embryogenesis was characterized by metric changes in egg parameters. At optimal temperature, the growth and development of eggs was accompanied by an increase in the egg and plugs' width with a simultaneous decrease in their length, as well as with thinning of the shell
The study presents species-specific morphological traits and metric parameters of mature male and female Сapillaria anatis (Nematoda, Capillariidae) nematodes. The capillariids were obtained by examinations of caeca of 205 domestic ducks (Anas platyrhynchos dom.), reared at poultry farms of Poltava, Sumy and Kyiv Regions of Ukraine. Noted sexual dimorphism was significant by seven morphometric parameters, with measurements of females being larger by 12.40–29.69 % than those of males. In addition to species-specific traits, other diagnostic characters were proposed in male and female С. anatis. In males, such taxonomically important features are morphological specifics of pseudobursa, spicule and ornamentation of spicule sheath. In identification of females, morphology of the vulvar area and eggs in uterus should be considered. Eleven metric parameters are suggested for better differential diagnostics of С. anatis.
The study presents species composition and abundance of nematodes of the family Capillariidae (Nematoda, Trichocephalida), parasitizing the domestic goose (Anser anser dom. Linnaeus, 1758) in Poltava region, Ukraine. Morphological specifics of adult specimens of collected helminths are examined and new data is obtained for species identification of Capillariidae considering their metric parameters. The pathogens of capillariasis in geese are represented by two species, Baruscapillaria anseris (Madsen, 1945) Moravec, 1982 and B. obsignata (Madsen, 1945) Moravec, 1982 with the former predominant. Prevalence of infection by B. anseris in domestic fowl was up to 46.8%, the abundance index reached 17.9 specimens. The proportion of geese infected with B. obsignata was 23.9%, and the abundance index was 3.8 specimens. Morphological study of the collected mature male and female nematodes revealed the significant similarity of both species in the general body structure and in identification characters: vulva and vagina structure in females, tail end, spicule and spicule sheath in males. Statistically significant differences were determined in the metric parameters of male and female B. anseris and B. obsignata nematodes. Those differences can be used to improve species identification. Males of B. anseris were larger than males of B. obsignata by 16 metric parameters including the length and width of body, spicule and spicule sheath, width of pseudobursa, and distance between the rays of pseudobursa. Females of B. anseris and B. obsignata were significantly different by seven metric parameters. By five parameters (length and width of various body regions) the B. anseris females were larger than B. obsignata. Metric parameters of the studied species are also of interest for taxonomic identification: the eggs of B. obsignata are longer and narrower than those of B. anseris.
214Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.
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