Cowpea (Vigna unguiculata) farmers from diverse geographical areas in northern Cameroon selected breeding lines from on-station trials for their own ®elds and explained their reasons (criteria) for making the selections. The average selection intensity employed by farmers varied from 6 to 17% and was similar to that employed by plant breeders. Their selection criteria fell into three broad categories relating to yield, preference and quality, and labour, which were very consistent over years, locations and gender. Selection criteria were strongly in¯uenced by market preferences, re¯ecting the increasing role of cowpea as a cash crop. Implications of these ®ndings for breeding strategies and genetic resource conservation are discussed.
Much of the proteolytic activity in the digestive tract of Callosobruchus maculatus larvae can be attributed to a thiol proteinase(s) that hydrolyzes 3H]methemoglobin by larval gut homogenates. Proteolytic activity in the larval gut was located in the lumen contents and thus appears to play a major role in extracellular digestion. The pH of the larval midgut is slightly acidic, and midgut contents exhibit a negative redox potential, conditions supporting the activity of a thiol proteinase. The significance of these findings is discussed with reference to the vulnerability of this digestive proteinase as a target for existing or genetically engineered plant chemical defenses.
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