<p class="Abstract">The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of <em>Berberis hispanica</em>. Three phenolic compounds (tamarixetin, caffeic acid and rutin)  were isolated from <em>B. hispanica</em>. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against <em>Klebsiella pneumonia</em> (15 mm at 100 mg/mL). Fraction T36 (IC₅₀&lt;5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC₅₀&lt;50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of <em>B. hispanica</em>.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Antiproliferative Assay: 4 min 31 sec   <a href="https://www.youtube.com/v/vi_fS47fpY0&amp;t=3s">Full Screen</a>   <a href="https://www.youtube.com/watch?v=vi_fS47fpY0&amp;t=3s">Alternate</a></p>

A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CHCl:MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CHCl:MeOH and 70% (v:v) MeOH:HO extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC = 14.0 ± 0.2 μg/mL) and fraction F5 (IC = 12.4 ± 0.1 μg/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC = 22.7 ± 0.6 μg/mL).

Objective: The aim of this study was to isolate the constituents and evaluate the antioxidant activity of Solanum rostratum Dunal (Solanaceae) from Algeria. Methods: In this study, phytochemical analyses of the chloroform, ethyl acetate, and n-butanol extracts obtained from the aerial parts of S. rostratum were performed by column chromatography, thin-layer chromatography, and high-performance liquid chromatography techniques. The antioxidant activity was performed by 2,2-diphenyl-1-picrylhydrazyl scavenging assay method.Results: The identification and structure elucidation of the isolated compounds were compared with their nuclear magnetic resonance spectra and the literature led to identify one monoterpene glycoside (linalyl-β-glucopyranoside) (1) and three flavonoid glycosides: Apigenin-7-O-glucoside (2), astragalin (3) and (isorhamnetin-3-O-glucoside (4).Conclusion: This is the first report on the isolation and structure elucidation of compound (1) (linalyl-β-glucopyranoside) and compound (2) (apigenin-7-O-glucoside) from this species. In addition, antioxidant effect of the chloroform extract from S. rostratum was evaluated. The chloroform extract exhibited the remarkable radical scavenging ability (IC50=0066±0.001 mg/mL).

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