Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria

Abstract

<p class="Abstract">The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of <em>Berberis hispanica</em>. Three phenolic compounds (tamarixetin, caffeic acid and rutin) were isolated from <em>B. hispanica</em>. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against <em>Klebsiella pneumonia</em> (15 mm at 100 mg/mL). Fraction T36 (IC<sub>50</sub><5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC<sub>50</sub><50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of <em>B. hispanica</em>.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Antiproliferative Assay: 4 min 31 sec <a href="https://www.youtube.com/v/vi_fS47fpY0&t=3s">Full Screen</a> <a href="https://www.youtube.com/watch?v=vi_fS47fpY0&t=3s">Alternate</a></p>

Phenolic Characterization, Antioxidant Activity, and Enzyme Inhibitory Properties of Berberis thunbergii DC. Leaves: A Valuable Source of Phenolic Acids

“…In previous literature, total bioactive components for several Berberis species have been reported. However, most studies were conducted on Berberis fruits [30,31,32]. A recent study reported that the methanol extract (80%) of Berberis orthobotrys contained more phenolics and flavonoids than the water extract [33], in agreement with our results.…”

Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from Berberis hispanica Boiss. & Reut

“…Therefore, we can confirm that the chemical content of the studied plant is strongly correlated to the biological outcomes. It was demonstrated that the rutin and tamarixetin contained in Berberis were responsible for the inhibition of cancer cell lines in a concentration-or time-dependent manner by inducing apoptosis and blocked cell cycle progression at the G2-M phase [10]. Antiproliferative activity in breast (MCF-7), colon (Caco-2), and pancreas (BxPC-3) cancer cell lines induced by Genus berberis was well reported elsewhere (21).…”

“…Fernández-Poyatos et al (2020) [12] reported that the most abundant compounds found in B. hispanica are phenolic acids, primarily chlorogenic acid and other caffeoylquinic acids. These compounds have been reported to have antioxidant effects [10,23]. Moreover, these compounds have also been shown to possess an in vivo and in vitro anticancer activity in various cancerous cell lines including MCF-7, HCT-116, Hep-G2, and PC-3.…”

“…The genus Berberis belongs to the family Berberidaceae with about 500 species. Berberis possesses many medicinal properties since it has been used in the treatment of diseases including leishmaniasis, heart disease, cholecystitis, hypertension, colds, cholelithiasis, dysentery, gallstones, digestive ailments, jaundice, malaria, ischemic heart disease, cardiomyopathies, and urinary tract problems [6][7][8][9][10]. The Spanish barberry is used in traditional medicine to cure gastrointestinal stones, inflammation, liver, and biliary disorders [11].…”

“…Genus Berberisis is rich in compounds including tamarixetin, rutin, caffeic, and chlorogenic acids with bioactivity potential. However, the available results on its chemical composition are more limited [10,12]. These compounds are responsible for Berberis biological activities such as antidepressant, antinociceptive, and immunomodulation effects [11,13].…”