Bioactive compounds from Eugenia  aromaticum were extracted by Soxhlet using DCM ,DCM;WATER,WATER and extracts were examined for its phytocomponents along with Clove oil. These bioactive plant compounds were screened for possible antimicrobial activities against two strains of MDR S. aureus. Antimicrobial activity of extracts using agar diffusion disc variant  method. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant were tested against two bacterial species,m Staphylococcus aureus ATCC 25923 and klebesella pnemonia ATCC 25922, by two variants of the agar diffusion method (well and disc), two variants of the bio autographic method (direct and indirect) and by microdilution assay. We concluded that the well-variant of the diffusion method was more sensitive than the disc-variant,  it was found extract 1 ie,DCM  and extract 2 i.e DCM;WATER had shown best results on antimicrobial activity ,phytocvhemical screening test are done for three extractsn invitro evalvation of antimicrobial activity was performed.

The leaves of Ocimum kilimandscharicum (privately known as Kapura Tulasi) have for some time been being used in the indian arrangement of medication for its enemy of diabetic properies.Could be a short herb, nearby to india it is used to treat various afflictions checking colds, hacks,stomach torments,measles and the runs.ocimum kilimandscharicum, has a spot to family lamiaceae .The current work , meant to assess in vitro enemy of diabetic action. Against diabetic appraisal was done by the boundaries, for example, glucose take-up by yeast cells, estimation of hemoglobin glycosylation, extricate impact at physiological glucose fixation. The standard medication utilized was Metronidazole and gallic corrosive. It indicated that EEOK in portion of 250µg/ml demonstrated high level of glucose take-up and hindered the hemoglobin glycosylation by expanding the grouping of hemoglobin. It additionally showed the hindrance of hemoglobin glycosylation at various physiological convergences of the glucose where the concentrate diminishes the development of the glucose-hemoglobin complex and in this manner measure of free hemoglobin increments. In view of the outcomes, it has been demonstrated that the EEOK displayed hostile to diabetic movement.

Ocimum kilimandscharicum is a short herb, native to India. In traditional medicine, it is used to treat various ailments including colds, coughs, abdominal pains, measles and diarrhea. Medicative plants, the “back bone” of ancient medication which implies over 3.3 billion folks within the less developed countries utilize medicative plants on an everyday basis. Ocimum kilimandscharicum Guerke (Syn. Ocimum camphora Guerke) belongs to family Lamiaceae. It is a native of Kenya and distributed in East Africa, India, Thailand, Uganda and Tanzania. It is extensively grown in the Tropics. In India it is cultivated on a small scale, especially in West Bengal, Assam, Tamil Nadu, Karnataka, Kerala and Dehradun. .The present study is aimed to evaluate in vitro anti-inflammatory, assessment was carried out on the basis of parameters such as heat induced protein denaturation, heat induced haemolysis, and Hypotonicity induced haemolysis. The standard drug was Diclofenac sodium. The findings of  the present study showed that the 500 µg/ml of Ethanolic extraction of Ocimum kilimandscharicum ( EEOK) showed more percentage inhibition when compared to standard. On the basis of results, it can be concluded that Ethanolic extraction of Ocimum kilimandscharicum (EEOK) showed significant anti- inflammatory activity.