The kinetics of antibacterial activity of cajuput oil (Melaleuca cajuputi) to E. coli, B. cereus, and S. aureus were investigated. The aim of this study was to determine the reaction orders of cajuput oil as an antibacterial agent. The extraction of cajuput oil was conducted by water-steam distillation. The yield was 0.88%. GC-MS analysis showed that the cajuput oil contained β-ocimene (19.35%), 1.8-cineole (17.67%), limonene (12.09%), β-caryophyllene (9.51%), γ-terpinene (8.93%), 2-β-pinene (8.85%), α-terpinolene (4.96%), α-humulene (4.10%), α-terpineol (2.83%), and p-cymene (2.33%). The extract showed antibacterial activity to E. coli, B. cereus, and S. aureus, with the reaction orders of 0.4460, 0.8235 and 0.6928, respectively.
Kinetic studies effectiveness clove leaf (Syzigium aromaticum) oil as antifungal Candida albicans have been done. The study have purpose to determine the reaction order, reaction constants (k) and relationship the concentration of clove leaf (Syzigium aromaticum) oil every time (At) with the initial concentration of of clove (Syzigium aromaticum) oil (Ao) and time (t) and equipped determination of the minimum concentration of clove leaf (Syzigium aromaticum)oil effective anti-fungus Candida albicans. The results shows the anti-fungal activity clove leaf (Syzigium aromaticum) oil on Candida albicans for each variation of the concentration 100%, 75%, 50% and 25% are 14.2 mm, 12.2 mm, 10.8 mm and 10.4 mm respectively. Reaction order as antifungal of the clove leaf (Syzigium aromaticum) oil on Candida albicans is 0.2112 with k = 5.0594. The minimum concentration of clove leaf (Syzigium aromaticum) oil as anti-fungal Candida albicans is 17.86%.
Antibacterial activity test Staphylococcus aureus and Salmonella typhihas been performed by microencapsulation product of rogo essential oil (Premnaserratifolia Linn). This study aims to determine rogo oil activity test and microencapsulation results as antibacterial S. aureus and S. typhi. The results of antibacterial activity of rogo liquid oil to S. aureus and S. Typhi bacteria showed different inhibitory power of each concentration variation of 12.5%, 25%, 50% and 100% with 100% concentration as the best inhibitor for both bacteria. While the antibacterial activity test of S. aureus and S. typhi from microencapsulated rogo oil: maltodextrin showed the difference of each variation of concentration 1:10; 1:12; 1:14; 1:16 and 1:18 with a 1:14 composition of S. aureus and 1:18 in S. typhi as the best ratio of activity power. The antibacterial activity test between liquid rogo oil and microencapsulated result shows the difference, but it can be concluded that the inhibitory power of rogo oil from microencapsulation is more inhibited than 100% rogo oil.
Research on the synthesis of methyl ester nitrate from nyamplung seed oil (Calophyllum inophyllum L.). The aims of study are to are to determine the nitrate methyl ester can be synthesized from nyamplung seed oil determine the yield of the transesterification and nitration reaction from nyamplung seed oil. In this study nyamplung seed oil was obtained from pressing nyamplung seeds using a hydraulic press, then the crude oil obtained was subsequently degumming to remove gum. Free oil of the etherified gum then transesterification with methanol. The transesterification results are then titrated and produce nitric methyl esters. Characterization of nitric methyl ester by spectrophotometer IR shows functional groups C-ONO2, C=C dan C-NO2 at wave number 1635,64 cm-1 1550,77 cm-1 dan 1365, 60 cm-1. The yield of transesterification of nyamplung oil was 64.66% and the nitration yield was 10.6%.
The phospholipids (PL) of pumpkin (Cucurbita moschata (Duch) Poir) seed kernel and their fatty acid composition were investigated. The crude oil was obtained by maceration with isopropanol followed by steps of extraction yielded polar lipids. The quantitative determination of PLs content of the dried pumpkin seed kernel and their polar lipids were calculated based on the elemental phosphorus (P) contents which was determined by means of spectrophotometric methods. PL classes were separated from polar lipids via column chromatography. The fatty acid composition of individual PL was identified by gas chromatography-mass spectrometry (GC-MS). The total of PL in the pumpkin seed kernels was 1.27% which consisted of phosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidyletanolamine (PE). The predominant fatty acids of PL were oleic and palmitic acid in PC and PE while PS's fatty acid were dominantly consisted of oleic acid and linoleic acid.
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