The Jumonji C (JmjC) domain-containing protein family, an important family of histone demethylase in plants, can directly reverse histone methylation and play important roles in various growth and development processes. In the present study, 51 JmjC genes (GhJMJs) were identified by genome-wide analysis in upland cotton (Gossypium hirsutum), which can be categorized into six distinct groups by phylogenetic analysis. Extensive syntenic relationship events were found between G. hirsutum and Theobroma cacao. We have further explored the putative molecular regulatory mechanisms of the JmjC gene family in cotton. GhJMJ24 and GhJMJ49 were both preferentially expressed in embryogenic callus compared to nonembryogenic callus in cotton tissue culture, which might be regulated by transcription factors and microRNAs to some extent. Further experiments indicated that GhJMJ24 and GhJMJ49 might interact with SUVH4, SUVH6, DDM1, CMT3, and CMT1 in the nucleus, potentially in association with demethylation of H3K9me2. Taken together, our results provide a foundation for future research on the biological functions of GhJMJ genes in cotton, especially in somatic embryogenesis in cotton tissue culture, which is crucial for the regeneration of transgenic plants.

Background Under the general trend of global aging, comorbidity among the elderly is becoming more and more common, which may have a certain impact on the quality of life of the elder. There is evidence that self-management and health empowerment are positively correlated with quality of life. However, limited research has explored the potential mechanism among them. Therefore, the purpose of this study is to explore the interaction path and mechanism among health empowerment, self-management and quality of life of elderly comorbid patients, and to provide theoretical basis for formulating intervention programs to improve their quality of life in the later stage. Objective To explore the relationship among health empowerment, self-management and quality of life of elderly patients with comorbidity. Methods 309 elderly patients with comorbidity in Zhengzhou City, Henan Province were investigated by questionnaire, including Health Empowerment Scale, Chronic Disease Self-management Scale and SF-12 Scale. Results The score of health empowerment, self-management and quality of life of the elderly patients with comorbidity were (92.18±8.917), (136.58±12.42) and (89.30±8.72) respectively. Health empowerment, self-management and quality of life of elderly patients with comorbidity were positively correlated (P < 0.01). The direct effect of health empowerment on quality of life is 0.493, the indirect effect is 0.207, the total effect is 0.724, and the intermediary effect accounts for 28.75% of the total effect. Self-management plays a partial intermediary role between health empowerment and quality of life. Conclusion The health empowerment, self-management and quality of life of elderly comorbid patients need to be further improved. The self-management of elderly comorbid patients plays a partial intermediary role between health empowerment and quality of life, and the quality of life of patients can be effectively improved by improving their health empowerment and self-management ability.

Filamin protein is characterized by an N-terminal actin-binding domain that is followed by 24 Ig (immunoglobulin)-like repeats, which act as hubs for interactions with a variety of proteins. In humans, this family has been found to be involved in cancer cell invasion and metastasis and can be involved in a variety of growth signal transduction processes, but it is less studied in plants. Therefore, in this study, 54 Filamin gene family members from 23 plant species were investigated and divided into two subfamilies: FLMN and GEX2. Subcellular localization showed that most of the Filamin gene family members were located in the cell membrane. A total of 47 Filamin gene pairs were identified, most of which were whole-genome copies. Through the analyses of cis-acting elements, expression patterns and quantitative fluorescence, it was found that GH_ A02G0519 and GH_ D02G0539 are mainly expressed in the reproductive organs of upland cotton, and their interacting proteins are also related to the fertilization process, whereas GH_A02G0216 and GH_D02G0235 were related to stress. Thus, it is speculated that two genes of the GEX2 subfamily (GH_A02G0519 and GH_D02G0539) may be involved in the reproductive development of cotton and may affect the fertilization process of cotton. This study provides a theoretical basis for the further study of the cotton Filamin gene family.