Highly virulent fowl aviadenoviruses (genus: Aviadenovirus) represent a significant risk in poultry farming that may contribute to increased mortality rates and may adversely affect the growth performance of poultry flocks. In this study, we performed the clinicopathological characterization of a FAdV strain SHP95 isolated from a commercial farm and its whole genome sequencing. The study revealed that the isolated strain is a highly virulent serotype 4 FAdV that can cause 100% mortality in day-old specific pathogen free (SPF) chickens with a dose of 2.5 × 10(5) TCID50. At a lower viral dose (1.5 × 10(4) TCID50), the infection in day-old SPF chickens caused 40% mortality and lesions characteristic for Hepatitis-hydropericardium syndrome (HHS). The viral strain was detectable by real time PCR in chicken organs, including the lymphoid organs until day 28 after infection. The whole genome assembly of strain SHP95 revealed a size of 45,641 bp, which encodes for 42 viral open reading frame (ORF). The comparative analysis in the genome shows 98.1% similarity between strain SHP95 and other FAdV-4 genomes reported. The major differences in the genome sequence between pathogenic and non-pathogenic fowl Adenovirus were identified in the right arm of the genome.

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From 2018 to 2019 symptoms consistent with infection by plant pathogenic phytoplasma bacteria were observed in commercial flower greenhouses located in Atlixco, Puebla, Mexico. Two varieties of Argyranthemum spp. plants exhibited symptoms of phyllody, causing serious concerns among farmers. To determine if phytoplasmas were associated with the symptomatic plants, the 16S rRNA-encoding locus (F2nR2 fragment) was amplified by PCR. This revealed that the symptomatic plants collected were infected by a phytoplasma belonging to the aster yellows group (16SrI-B). These results were confirmed and refined by amplification and sequencing of the phytoplasma chaperonin 60 universal target (cpn60 UT). Sequence analysis using the online tool CpnClassiPhyR confirmed that the phytoplasma detected is a member of the 16SrI group, specifically a member of the cpn60 UT I-IB subgroup.. This is the first report of daisies affected by phytoplasma in Mexico, and the first report of the use of CpnClassiPhyR to characterize an unknown phytoplasma strain. The detection of phytoplasmas infecting commercially produced daisies and their characterization provides an impetus for phytoplasma management in Atlixco, a Mexican city for which the production of ornamental plants and cut flowers is of economic importance. KeywordsAster yellows phytoplasma • Flowers • 16SrI-B • Atlixco • CpnClassiPhyR Phytoplasmas ('Candidatus Phytoplasma' spp.) are insect-transmitted bacteria limited to the phloem and have been associated with a wide array of diseases in many ornamental and food crop plants (Pérez-López et al. 2016a). Daisies, chrysanthemums, and other flowers within the family Asteraceae are susceptible to phytoplasma infection