The stability of various formulations of mayonnaise stored in commercial glass jars, at 20C in the dark was studied. Dijon mayonnaise made with sunflower oil and containing mustard paste can be stored up to 10 months in closed jars without serious oxidative damage due to the presence of high amounts of natural tocopherols in the oil and limited oxygen availability. However, in the absence of mustard, the oxidative degradation was somewhat faster and the amount of conjugated dienes was increased more quickly and to a higher degree than that in the mustard containing sample. Supplying oxygen by opening one time the jars to air increased the rate of degradation, even when the mayonnaise was stored at 4C. Exposing of glass jars of mayonnaise alternatively to daylight and darkness for 1 month produced only little conjugated dienes while hexanal appeared in significant amounts. Addition of antioxidant (rosemary extract and EDTA) or emulsifier (DATEM) decreased the level ofphotooxidative volatiles in the headspace.
During cocoa beans roasting, there are physicochemical changes that develop the chocolate quality attributes. Roasting systems have a particular influence on the development of these characteristics, and the effects of operation variables for each system must be evaluated. The objective of this study was to evaluate the effect of roasting time and temperature in a rotatory system on cocoa beans physicochemical parameters of quality as moisture, water activity, pH, total acidity, color ( * , * , * ), total phenolic content (TPC), and DPPH radical capacity. Cocoa beans were roasted as a function with a central rotatable design with 2 2 + 5 central points and 4 axial points (−1.414, −1, 0, +1, and +1,414) and a response surface methodology was applied. Temperature and time levels were 110-170 ∘ C and 5-65 minutes, respectively. The effect of the variables was nonlinear and modeled with a second-order response polynomial. Roasting time and temperature presented a significative effect ( < 0.05) on the response variables except for both TPC and DPPH radical capacity in aqueous extract.
Oil-in-water emulsions were prepared with 30% stripped sunflower oil, stabilized by 20 g/L BSA and homogenized under high pressure to obtain a mean droplet size near 0.5 µm. The emulsions were shown to be physically stable during storage in a shaker at 47°C for 5 d. Such a medium was suitable to test the efficiency of different types of antioxidants. Oxidation of control emulsions appeared rapidly without a lag phase, and the contents of conjugated dienes and hexanal reached a plateau after around 20 h. In the presence of EDTA, the oxidation was strongly inhibited, suggesting that some metallic ions present in the oil or the protein solution act as inducers. Ascorbic acid and ascorbyl palmitate were inactive. Isoeugenol was found to be a powerful antioxidant, better than eugenol, α-tocopherol, and Trolox.
The cocoa shell is a residue of low commercial value, which represents an alternative for obtaining substances of added value for the food and pharmaceutical industry. Substances of interest in the shell include fat and methylxanthines (theobromine and caffeine). In order to obtain the extraction behavior with supercritical CO2, a 23 factorial design was proposed with six central points, taking dynamic extraction into consideration. The following factors were involved: pressure (2,000–6,000 psi), temperature (313–333 K), and time (30–90 min). The obtained yield was between 3.66% and 15.30%. Fat was the substance that was extracted most effectively (94.73%). Caffeine demonstrated variability in the residue, with at least six treatments that exceeded a removal rate of more than 90%, while it was practically impossible to extract theobromine. The difference with regard to the extraction of theobromine may be attributed to its low solubility. Characterization using FT–IR showed the modifications before and after the process, providing clear evidence of the changes corresponding to the fat at 2,924, 2,854 and 1,745 cm−1. The results presented establish the basis for the extraction of substances such as fats and methylxanthines from a cocoa shell with the use of CO2.
Protein isolates prepared by alkaline solubilization followed by isoelectric precipitation and freeze-drying from six varieties of Lupinus angustifolius (Haags Blaue, Sonate, Probor, Borlu, Boregine, and Boruta) grown in Mexico were evaluated for functional properties: nitrogen solubility, water-holding capacity (WHC), oil holding capacity (OHC), emulsion activity index (EAI), emulsion stability index (ESI), foaming capacity (FC), foam stability (FS), and gelling minimum concentration (GMC). The nitrogen solubility values, WHC, OHC, and FC did not show significant differences between the protein isolates. The solubility of the isolates was minimal at pH of 4.0 and 5.0 while the regions of maximum solubility were found at pH of 2.0 and 10.0. There were significant differences in EAI and ESI depending on the varieties used. The isolates of the Boregine and Borlu varieties showed the highest EAI with 29.3 and 28.3 m 2 g −1 , respectively, while the lowest index was recorded in the isolate obtained from the Sonate variety (24.6 m 2 g −1 ). Like solubility, these indices also increased at both extremes of pH evaluated; both properties were minimal in the isoelectric pH range (4.0 to 5.0).
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