No abstract

Investigation of mitochondrial morphology and function has been hampered because photostable, mitochondrion-spZc stains that are retained in fted, permeabilized cells have not been available. We found that in live cell preparations, the CMXRos and H2-CMXRos dyes were more photostable than rhodamine 123. In addition, fluorescence and morphology of mitochondria stained with the CMXRos and ation and acetone permeabilization. Using epifluorescence microscopy, we showed that CMXRos and H2-CMXRos dye fluorescence fully co-localized with antibodies to subunit I of cytochrome c oxidase, indicating that the dyes specifically stain mitochondria. Confocal microscopy of these mitochondria yielded colored banding pattems, suggesting that these dyes and the mitochondrial enzyme localize to different suborganellat regions. Therefore, these stains provide cMxR0s-H~

No abstract

No abstract

No abstract