This study investigated the flux stability of select chemical and biological sewage markers, including caffeine, total nitrogen (TN), total suspended solids (TSS), E. coli, and enterococci, and their suitability in assessing the severity of rainfall-derived infiltration and inflow (RDII) in a residential sewershed. To quantify and compare marker flux stability, concentrations of the candidate markers in two dry-weather periods were determined and the one-day lag autocorrelation coefficients (r) of their mass fluxes were calculated. TN (r = 0.82-0.88) exhibited higher and more consistent flux stability than TSS (r = 0.49-0.82), caffeine (r = 0.56-0.58), E. coli (r = 0.36-0.87), and enterococci (by culture; r = 0.40-0.52), all of which except enterococci by qPCR (r = -0.10-0.21) showed significant autocorrelation. Sewage flows and marker concentrations were also monitored in two wet-weather periods, and the severity of RDII (R(RDII)) were calculated using either flow measurements or marker concentrations independently. Corresponding to its outstanding flux stability, R(RDII) values estimated by TN predicted all severe RDII instances and gave the highest and most consistent correlation (r = 0.74-0.78) among the different sewage markers. Overall, the study illustrated the feasibility of using the flux stability of sewage markers in assessing the severity of RDII and thereby deterioration levels in sewer systems.

The increase of swine production in the Pacific Islands has inevitably led to environmental pollution concerns from discharged wastewater derived from both washing and manure. The slurry accumulates in lagoons, where supernatant wastewater containing high levels of pathogens and nutrients becomes nonpoint source water pollution that deteriorates the quality of receiving water bodies. Soil filtration is a promising cost-effective technology for removing pollutants from swine wastewater; however, the excessive growth of bacteria in soil media often accompanies the filtration process. This study investigates soil filtration mediated by protozoa activities to remove Escherichia coli (E. coli) in synthetic swine wastewater. The experiment used plastic columns packed with Leilehua soil from Oahu Island, Hawaii. The soil physicochemical adsorption was seen to reduce 95.52%–96.47% of E. coli. However, the average removal efficiencies were increased to 98.17% in a single stage, and 99.99% in two sequential columns, under predation conditions. The filtration media containing naturally established bacterivores with the prey, provided a bioactive means to remove E. coli from the influent. The proper design of Leilehua soil filters potentially removes E. coli from the influent to meet the standard level of recycled water.

Nghiên cứu này nhằm đánh giá hiệu suất của đất ngập nước (ĐNN) nhân tạo dòng chảy ngầm theo phương ngang (HSSF) và phương đứng (VF) có vật liệu nền là xỉ than tổ ong và trồng cỏ voi. Thí nghiệm được tiến hành trên mô hình phòng thí nghiệm với lưu lượng nạp của nước thải sinh họat là 85 lít/ngày. Tải lượng nạp BOD5, COD, TN, TP vào mô hình lần lượt là 7,47 g/m2.ngày, 3,17 g/m2.ngày, 1,43 g/m2.ngày, 0,12 g/m2.ngày. Kết quả nghiên cứu cho thấy nồng độ các chỉ tiêu ô nhiễm sau xử lý giảm đáng kể và đạt loại A theo QCVN 14:2008/BTNMT trong cả hai mô hình HSSFCW và VFCW. Hiệu suất xử lý của HSSFCW và VFCW đối với các chỉ tiêu lần lượt là SS: 88,7% và 92,4%; BOD5: 95,3% và 92,6%; COD: 94,3% và 92,6%; TN: 54,1% và 47,5%; N-NO3-: 38,4% và 33,6%; TP: 73,5% và 63,2%; P-PO43-: 87,6% và 59,7%. Nhìn chung, mô hình HSSFCW có hiệu suất loại bỏ các chất ô nhiễm tương đối cao hơn mô hình VFCW, ngoại trừ chỉ tiêu SS. Cỏ voi phát triển tốt và cho sinh khối cao trong thí nghiệm. Từ kết quả nghiên cứu cho thấy xỉ than tổ ong có thể tái sử dụng làm chất nền trong ĐNN nhân tạo dòng chảy ngầm. Bên cạnh đó, cỏ voi có thể trồng trong hệ thống ĐNN dòng chảy ngầm xử lý nước thải sinh hoạt. ABSTRACT This study aimed to evaluate the performances of horizontal subsurface flow (HSSF) and vertical flow (VF) constructed wetlands (CW) using combusted beehive charcoal residues as filtration bed media and planted with Napier grass (Pennisetum purpureum). The experimental systems were fed with a flow rate of 85 m3/day. The loading rates of BOD5, COD, TN, TP into the system were 7.47 g/m2.day, 3.17 g/m2.day, 1.43 g/m2.day, 0.12 g/m2.day, respectively. The results showed that the concentration of pollutants in effluents is significantly reduced and meet the national standard type A of QCVN 14:2008/BTNMT in both HSSFCW and VFCW models. The removal efficiencies in HSSFCW and SVFCW for SS: 88.7% and 92.4%; BOD5: 95.3% and 92.6%; COD: 94.3% and 92.6%; TN: 54.1% and 47.5%; N-NO3-: 38.4% and 33.6%; TP: 73.5% and 63.2%; P-PO43-: 87.6% and 59.7%, respectively. In general, the HSSFCW model has a relatively higher pollutant removal efficiency than the VFCW model, except for the SS. good growth and high biomass yield of Napier grass had been observed in the experimental systems. This study suggested that combusted beehive charcoal residues could be reused as bed substrate in constructed wetlands. Besides, Napier grass might also be a potential plant associated with subsurface flow constructed wetlands to treat domestic wastewater.