No abstract

Bacterial associated infection is a threat in the medical field. Pseudomonas aeruginosa, one of the major causative agents for nosocomial infection, has developed resistance to almost all the classes of antibiotics. Recently, nanopillar-like structures were identified on the wings of insects such as cicada and dragonfly. Nanopillars both on natural surfaces and those mimicked on artificial surfaces were reported to possess bactericidal activity against a wide range of bacteria. An earlier study reported strain specific variation in the viability of P. aeruginosa on the nanopillar topography of a dragonfly wing under static condition. Here, we report the behavior of P. aeruginosa strains on a dragonfly wing under hydrodynamic conditions. The results of the study indicated that, under hydrodynamic conditions, P. aeruginosa PAO1 was attached in higher numbers to the wing surface than P. aeruginosa ATCC 9027 but killed in lower numbers. The plausible reason was identified to be the masking of nanopillars by the secreted extracellular polysaccharide. The shear rate of 1.0 s−1 showed a relatively higher bactericidal effect among the three tested shear rates.

Purpose Fungal rhinosinusitis is on the rise worldwide and it is endemic especially in North India. The main purpose of this study was to determine the antifungal resistance profile of fungal isolates from the cases of fungal rhinosinusitis. Methods Antifungal susceptibility testing of isolated fungi to fluconazole, amphotericin B, itraconazole, and voriconazole was determined by standard CLSI broth microdilution method. Results Sixty-eight fungal isolates of Aspergillus spp. (n = 49), Rhizopus spp. (n = 9), Candida spp. (n = 4), Penicillium spp. (n = 2), Mucor spp. (n = 2), Bipolaris spp. (n = 1), and Alternaria spp. (n = 1) were obtained from 60 different clinical samples as exudate from nasal mucosa (n = 28), allergic mucin (n = 8), nasal lavage (n = 2), tissue biopsy from nasal polyps (n = 14), and intraoperative nasal mucosa (n = 8). Of the 68 isolates, 75% were resistant to fluconazole, 13.23% were resistant to itraconazole, 2.94% to amphotericin B, and none were resistant to voriconazole. Aspergillus flavus (5%) was the only fungi found resistant to amphotericin B, while against itraconazole, A. flavus (7.5%) and A. niger (100%) were found resistant. All the isolates of A. flavus, A. fumigatus, A. niger, and Penicillium spp. were resistant to fluconazole. Conclusion Although amphotericin B stills remains to be the most effective drug, more prospective studies are needed for the requirement of knowledge of the sensitivity pattern for optimal treatment and reduction in morbidity in the long run.

Introduction: Non typhoidal Salmonella (NTS) causes urinary tract infections infrequently and are usually associated with presence of genitourinary abnormalities. Case Presentation: We report a case of immunocompetant male in his early 20s with phimosis presented with history of dysuria and burning micturition for 4 months. A renal ultrasonography showed presence of bilateral intrarenal calculi. Urine analysis revealed presence of non typhoidal Salmonella. Automated identification systems performed poorly in identification of serotype. On serotyping, it was identified as Salmonella enteritidis in the referral centre. The patient was managed with oral antibiotics. Conclusion: This report highlights the issues inaccurate identification of NTS even with advanced automated systems and early initiation of therapy based on the knowledge of local susceptibility patterns. Furthermore, UTI in immunocompetent individuals by non typhoidal salmonellae should always be investigated further to rule out genitourinary abnormalities and appropriate antibiotics must be started to avoid chronicity and complications.