Abstract-With the fast growing demand of location-based services in indoor environments, indoor positioning based on fingerprinting has attracted a lot of interest due to its high accuracy. In this paper, we present a novel deep learning based indoor fingerprinting system using Channel State Information (CSI), which is termed DeepFi. Based on three hypotheses on CSI, the DeepFi system architecture includes an off-line training phase and an on-line localization phase. In the off-line training phase, deep learning is utilized to train all the weights of a deep network as fingerprints. Moreover, a greedy learning algorithm is used to train the weights layer-by-layer to reduce complexity. In the on-line localization phase, we use a probabilistic method based on the radial basis function to obtain the estimated location. Experimental results are presented to confirm that DeepFi can effectively reduce location error compared with three existing methods in two representative indoor environments.

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Cholesterol oxidase (COD), an enzyme catalyzing the oxidation of cholesterol, has been applied to track the distribution of membrane cholesterol. Little investigations about the effect of COD on tumor cells have been performed. In the present study, we provided evidence that COD from Bordetella species (COD-B), induced apoptosis of lung cancer cells in vitro and in vivo. COD-B treatment inhibited Akt and ERK1/2 phosphorylation in dose- and time-dependent manner, which was not reversed and was even aggravated by cholesterol addition. Further investigation indicated that COD-B treatment promoted the generation of reactive oxygen species (ROS) and that cholesterol addition further elevated ROS levels. Moreover, COD-B treatment resulted in JNK and p38 phosphorylation, downregulation of Bcl-2, upregulation of Bax, activated caspase-3 and cytochrome C release, which likely responded to freshly produced hydrogen peroxide that accompanied cholesterol oxidation. Catalase pretreatment could only partially prevent COD-B-induced events, suggesting that catalase inhibited H2O2-induced signal transduction but had little effect on signal pathways involved in cholesterol depletion. Our results demonstrated that COD-B led to irreversible cell apoptosis by decreasing cholesterol content and increasing ROS level. In addition, COD-B may be a promising candidate for a novel anti-tumor therapy.