Respiratory syncytial virus (RSV) is a leading cause of hospitalization in infants. A formalin-inactivated RSV vaccine was used to immunize children in 1966 and elicited non-protective, pathogenic antibody. Two immunized infants died and 80% were hospitalized after subsequent RSV exposure. No vaccine was licensed since. A widely accepted hypothesis attributed vaccine failure to formalin disruption of protective antigens. Instead, we show that lack of protection was not due to alterations caused by formalin, but to low antibody avidity for protective epitopes. Lack of antibody affinity maturation followed poor Toll-like receptor stimulation. This study explains why the inactivated RSV vaccine failed to protect and consequently led to severe disease, hampering vaccine development for forty-two years. Also, it suggests that inactivated RSV vaccines may be rendered safe and effective by inclusion of TLR-agonists in their formulation. In addition, it identifies affinity maturation as a critical factor for the safe immunization of infants.

A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assays showed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV. Differences between the diagnostic tests and their choice according to the age of the animal are discussed. The clinical histories of ninety of the 255 cats showed blood profiles similar to others previously reported and revealed a higher risk of infection in male adult cats with outdoor access.

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The World Climate Research Programme (WCRP) envisions a world “that uses sound, relevant, and timely climate science to ensure a more resilient present and sustainable future for humankind.” This bold vision requires the climate science community to provide actionable scientific information that meets the evolving needs of societies all over the world. To realize its vision, WCRP has created five Lighthouse Activities to generate international commitment and support to tackle some of the most pressing challenges in climate science today. The overarching goal of the Lighthouse Activity on Explaining and Predicting Earth System Change is to develop an integrated capability to understand, attribute, and predict annual to decadal changes in the Earth system, including capabilities for early warning of potential high impact changes and events. This article provides an overview of both the scientific challenges that must be addressed, and the research and other activities required to achieve this goal. The work is organized in three thematic areas: (i) monitoring and modeling Earth system change; (ii) integrated attribution, prediction and projection; and (iii) assessment of current and future hazards. Also discussed are the benefits that the new capability will deliver. These include improved capabilities for early warning of impactful changes in the Earth system, more reliable assessments of meteorological hazard risks, and quantitative attribution statements to support the Global Annual to Decadal Climate Update and State of the Climate reports issued by the World Meteorological Organization.