Escherichia coli O157:H7 is frequently detected in ready-to-eat produce and causes serious food-borne diseases. The decontamination efficacy of lactic acid (LA) is clearly established. In this study, LA was mixed with acetic acid (AA) to reduce costs while achieving consistent or better inhibitory effects. Time-kill curves and inoculation experiments using fresh-cut spinach and arugula indicated that 0.8%LA+0.2%AA shows similar antibacterial effects to those of 1%LA. To determine whether 1%LA and 0.8%LA+0.2%AA exert antibacterial effects by similar mechanisms, proteomics analysis was used. The proteins related to macromolecule localization, cellular localization, and protein unfolding were uniquely altered after the treatment with 1%LA, and the proteins related to taxis, response to stress, catabolic process, and the regulation of molecular function were uniquely altered after the treatment with 0.8%LA+0.2%AA. Based on these findings, combined with the results of a network clustering analysis, we speculate that cell membrane damage is greater in response to LA than to 0.8%LA+0.2%AA. This prediction was supported by cell membrane permeability experiments (analyses of protein, nucleotide, ATP, and alkaline phosphatase leakage), which showed that LA causes greater membrane damage than 0.8%LA+0.2%AA. These results provide a theoretical basis for the application of an acid mixture to replace LA for produce decontamination.

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In order to explore the hazard analysis critical control point (HACCP, an internationally recognized food safety detection system) effect on food quality in liquor production, affecting the liquor five hazards monitoring, determine the key point is under control and discusses the relevant follow-up work, determine the HACCP work-flow, and discusses the implementation of HACCP, faithfully record the production process, put forward the critical limits, and strict monitoring, ensure the safety of liquor products stability, provide a reference for liquor enterprise production management.

To prolong the storage period of fresh Lilium longiflorum, maintain its quality during storage, and optimise the controlled atmosphere (CA) storage parameters. In a single-factor experiment, the temperature, humidity, and O2 and CO2 concentrations were considered as primary factors affecting the CA storage of L. longiflorum. Then, a comprehensive score was optimised using a back propagation neural network combined with empirical data. Finally, experimental verification was undertaken. The optimal concentrations of O2 and CO2 were 4.5% and 3.8%, respectively. A storage temperature of 4.2°C and a relative humidity of 90% were ideal. Under these conditions, the comprehensive evaluation score for L. longiflorum was 0.8424 (P > 0.05), consistent with the predicted value of 0.8372. Compared to ordinary cold storage, the storage period of L. longiflorum under these CA storage conditions was effectively prolonged. This provided an experimental basis for the CA storage of L. longiflorum.