Background. World Health Organization pointed out in 2019 that insufficient physical activity has become the fourth major risk factor affecting global mortality. Objectives. This research explores the influence of multi-media teaching intervention in the physical education curriculum on college students' sports attitudes and behavior. Methods. The subjects of weight training and Yogalates courses (94 people in weight training class 2 and 94 people in Yogalates class 2) were divided into experimental and control groups. Both groups were taught in person for 18 weeks. The experimental group participated in multi-media audio-visual teaching for 6 weeks, once a week, for 30 minutes. The control group did not implement multi-media audio-visual teaching. Before and after the teaching experiment, 188 questionnaires were issued with the sports attitude and sports behavior scales, and 184 (97.8%) were effectively returned in the pre-test; 180 (95.7%) were effectively returned in the post-test. After the questionnaire was collected, it was analyzed by descriptive statistics, cross-analysis, and an independent sample t-test. Results. The study found that after multi-media teaching is involved in physical education courses, students' sports attitudes and behaviors are significantly higher than in traditional teaching, and the number of students participating in sports after class has increased significantly. Conclusion. Multi-media teaching intervention in physical education courses can effectively improve students' attitudes towards sports, change their actual participation in sports, and enable students to develop the habit of continuing to participate in sports after class.