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SummaryA method for quantitation of fiprin deposits in organs of rats, using labelled fibrinogen and albumin, is described. In rats previously injected with 125I-fibrinogen, thrombin was used to induce intravascular deposition of fibrin in various organs. 131I-albumin was injected shortly before sacrifice for determination of the organ plasma volume. The fibrin content of the organ was calculated by subtraction of the plasma and extravascular 125I-radioactivities from the total 125I-content of the organ. The organ plasma radioactivity was obtained as the product of 12 5I in plasma and the organ plasma volume and the extravascular radioactivity as the difference between total and plasma radioactivity in organs of rats before the thrombin infusion. It was found that the method is especially suited for quantitation of fibrin deposition and elimination in the lungs, but it should be also applicable to other organs. In the liver and kidneys certain problems occur which interfere especially with fibrin elimination studies in these organs.

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In 29 cases of sudden death the insulin concentration in blood from the right heart and the femoral vein was determined by a radioimmunological method. The concentrations in the femoral venous blood were below 60 muU/ml serum (mean 23 muU/ml), i.e. in the same order of magnitude as in living persons. In right heart blood the insulin values were about 10 times higher, probably due to post-mortal diffusion of insulin via the portal vein. In suspected hyperinsulinism the measurements should therefore be made on peripheral venous blood.