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Because of its simplicity the method is well suited for determining the inhibition of aromatic amino acid decarboxylase in vivo. It has the additional advantage of measuring the effect of decarboxylase inhibitors in situ rather than in homogenates prepared from a single organ. The method in its present form does not assay the inhibition of decarboxylase in the central nervous system.

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A high-performance liquid chromatographic (HPLC) method has been developed for the quantitative determination of salinomycin in chicken skin/fat. For this procedure skin/fat homogenate (10 mL, equivalent to 2 g of tissue) was extracted with methanol. The extract was partitioned with carbon tetrachloride, applied to a silica gel column, and, in turn, run through a C18 column. The purified salinomycin solution was then oxidized with pyridinium dichromate and washed with sodium bicarbonate. The derivatized product was purified by running it through a silica gel column. The HPLC system utilized an automated on-line column-switching system with UV detection at 225 nm; the minimum limit of detection was 100 ppb. Peak height ratios of salinomycin to internal standard were used for quantitation.

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