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Acanthamoeba infection occurred in a 4-year-old military working dog (German shepherd). The disease was manifested by multifocal necrohemorrhagic foci in the heart, lungs, liver, and pancreas. Numerous organisms morphologically compatible with Acanthamoeba sp. were seen in the lesions. The infection may have been caused by local invasion of a wound and hematogenous spread, via the respiratory tract, by inhalation of the organisms, or by direct extension of an inapparent nasal infection.

Abstract. Pulmonary granulomas were incidental findings at necropsy in 18 dogs, mainly of the brachycephalic breeds. These lesions were multifocal along smaller bronchi and the subpleural tissue at the margins of lobes. The granulomatous reaction contained numerous periodic acid-Schiff (PAS)-positive bodies that histochemically had reactions suggestive of a calcium-lipid-mucopolysaccharide complex. Although the cause is not clear, the location and distribution are suggestive of an inhalation or aspiration pneumonia.The shortened muzzle of brachycephalic breeds, particularly when there is concurrent upper respiratory disease, may predispose them to these bodies.Pulmonary granulomas of unknown cause were seen at necropsy as incidental findings in 18 dogs with a mean age of 10 years. All but two dogs were from large metropolitan areas of New York City and Philadelphia. Tissue specimens were submitted to the Armed Forces Institute of Pathology for histologic examination. The gross and histologic features of these lesions are described. Materials and MethodsThe breed, sex, and age of each dog is shown in table I. Parts of the pulmonary lesions were fixed in 10% formalin and embedded in paraffin. Sections were cut at 6 pni and stained with haematoxylin and eosin (HE), periodic acid-Schiff (PAS) with and without diastase, Mowry's modification of the colloidal iron stain for acid mucopolysaccharides (AMP) [4], alizarin red S before and after microincineration, Danielli's stain for tyrosinecontaining proteins, alcian blue, oil red 0, and the Prussian blue reaction for iron. Other stains included Congo red for amyloid, Mayer's mucicarmine, and Gomori's methenaminesilver stain (GMS). X-ray diffraction was performed by the powder method and a DebyeScherrer camera [l].