ABSTRACT. This study evaluated the effects of the autosomal dominant Fm gene in conjunction with the sex-linked Id gene on skin color and related gene expression. Ten Dongxiang black cocks were selected to build ten families by mating 60 individuals of ISA B-line layers. The skin color of the F 1 generation was observed at different time points. At 126 days, 36 chickens were slaughtered, and gene expression of TYRP1, TYRP2, MC1R, and EDNRB in breast skin was assessed by quantitative RT-PCR. The ratio of Dongxiang black chickens with white skin chicks in the F 1 generation to that of non-white was 3:7 (HoFF: HeFf). At 126 days, all F 1 generation cocks showed white skin (115/115), while the percentages of hens with black skin were 100% (HoFF, 27/27) and 53.75% (HeFf, 43/80). The change in skin color peaked between 42 and 84 days. The offspring of HoFF displayed significantly higher expression of MC1R, compared with those of HeFf (P < 0.05). The "L" value of hen's skin was significantly lower, and TYRP1 and TYRP2 expression was significantly higher (P < 0.05) than in cocks with the same Fm/ fm genotype. These findings indicate the presence of homozygous and heterozygous Fm in Dongxiang black chickens, with the offspring of X.D. Zhang et al. 11552©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11551-11561 (2015) homozygous birds showing a higher percentage of black skin percentage. The expression of the four genes studied was correlated with skin color, with TYRP1 and TYRP2 representing the most suitable molecular markers.

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ABSTRACT. Lunella coronata granulata, from the family Turbinidae, is an economically important species. The first set of 10 polymorphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559. Eight loci were in Hardy-Weinberg equilibrium (HWE) (P > 0.05), while two loci deviated significantly from the HWE after Bonferroni's correction (P < 0.005). The isolated microsatellite loci can be utilized in studies of population genetic analysis and they provide important genetic markers for construction of genetic linkage maps and genetic breeding of L. coronata granulata resources.

ABSTRACT. Megalonibea fusca is a commercially important large edible fish. In this study, the first set of 10 polymorphic microsatellite loci for M. fusca was developed and characterized. The number of alleles per locus ranged from two to five, with the observed and expected heterozygosities ranging from 0.0667 to 0.7667, and from 0.0644 to 0.5828, respectively. Most of the loci were in HardyWeinberg equilibrium (P > 0.05), except for two loci (Mf25 and Mf30) after a Bonferroni's correction (P < 0.005). These informative microsatellite markers will be useful in further studies of the population and conservation genetics of this species.