We report a novel label-free method for the investigation of the adaptive recognition of small molecules by nucleic acid aptamers using capillary electrophoresis analysis. Cocaine and argininamide were chosen as model molecules, and the two corresponding DNA aptamers were used. These single-strand DNAs folded into their specific secondary structures, which were mainly responsible for the binding of the target molecules with high affinity and specificity. For molecular recognition, the nucleic acid structures then underwent additional conformational changes, while keeping the target molecules stabilized by intermolecular hydrogen bonds. The intrinsic chemical and physical properties of the target molecules enabled them to act as indicators for adaptive binding. Thus any labeling or modification of the aptamers or target molecules were made obsolete. This label-free method for aptamer-based molecular recognition was also successfully applied to biological fluids and therefore indicates that this approach is a promising tool for bioanalysis.

In order to give an in-depth understanding of the contradictions arising from the land resource supply and demand, this study selected 30 provinces (some are autonomous regions or municipalities) in China to be the research unit, used the carbon emission as an undesirable output, and adopted the Super-SBM DEA model and ESDA-GWR method to research the evolution characteristics and influencing factors of land use efficiency in China in 2003–2013. The results indicated that: (1) The land use efficiency in China overall was moderately ineffective and the overall utilization level was low; (2) The Global Spatial Autocorrelation was instable and had maintained a high level; (3) The “hot spots” mainly being distributed in the southeast coastal regions and “cold spots” being found in the central and western regions, so that as time goes on, the pattern of “high in the east and low in the west” has been gradually formed and stabilized. (4) The GWR model analysis showed that the natural factors such as NDVI, DMSP/OLS and DEM have a significant impact on land use efficiency, thereby providing an important contribution to this study. For the eastern coastal areas, the emphasis should be improving their OT, PF and PGDP, for the western region, should focus on improving its comprehensive economic development level to improve the DMSP/OLS, while strengthening the ecological environment to improve the level of NDVI.

With business today relying increasingly more on collaboration, new product development is also on a network base. The concept of innovation ecosystem is built upon knowledge creating and sharing across companies, knowledge institutions, policy regimes, business enterprises and industry boundaries. China as the largest emerging market has witnessed innovation with interaction among government, university, industry and research. Though government plays an important role in promoting innovation, not many studies have covered the detailed dynamic process and impact of policy on forming innovation ecosystem, especially in places where initial knowledge resources such as universities and research institutes are limited. Therefore, our article aims to fill in this research gap. Through document review and case study on Changzhou region of China, 1 we map out the local government policy concerns and changes during each stage of innovation ecosystem formation, development and expansion from 2001 to 2015. The interaction mechanism among government, university, industry and research is summarised with a proposed framework to highlight the key policy making areas. Further research areas are recommended with implication and conclusion.