ObjectivesTo elucidate the effects of age on the expression levels of the receptor activator of the nuclear factor-κB ligand (RANKL) and osteoclasts in the periodontal ligament during orthodontic mechanical loading and post-orthodontic retention.Materials and MethodsThe study included 20 male Sprague-Dawley rats, ten in the young group (aged four to five weeks) and ten in the adult group (aged 18 to 20 weeks). In each rat, the upper-left first molar was subjected to a seven-day orthodontic force loading followed by a seven-day retention period. The upper-right first molar served as a control. The amount of orthodontic tooth movement was measured after seven-day force application and seven-day post-orthodontic retention. The expression levels of RANKL and the tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts were evaluated on day 7 (end of mechanical force loading) and day 14 (after seven days of post-orthodontic retention). Statistical analysis was performed using the t-test, and significance was set at p < 0.05.ResultsThere was no significant difference between the amount of tooth movement in the young group (0.96, standard deviation (sd) 0.30mm) and that in the adult group (0.80mm, sd 0.28) (p > 0.05) after the seven-day force application. On the compression side, the expression of RANKL and TRAP-positive osteoclasts in both the young and the adult groups increased after the application of force for seven days, and then decreased at the end of the seven-day retention period. However, by the end of the period, the expression of RANKL on the compression side dropped to the control level in the young group (p > 0.05), while it was still higher than that on the control side in the adult group (p < 0.05). The expression of RANKL on the compression side did not show significant difference between the young and the adult groups after seven-day force application (p > 0.05), but it was significantly higher in the adult group than that in the young group after seven-day post-orthodontic retention (p < 0.05). Similarly, the decreasing trend of TRAP-positive osteoclasts during the retention period in the adult group was less obvious than that in the young group.ConclusionsThe bone-resorptive activity in the young rats was more dynamic than that in the adult rats. The expression of RANKL and the number of osteoclasts in adult rats did not drop to the control level during the post-orthodontic retention period while RANKL expression and the number of osteoclasts in young rats had returned to the baseline.Cite this article: X. Li, M. Li, J. Lu, Y. Hu, L. Cui, D. Zhang, Y. Yang. Age-related effects on osteoclastic activities after orthodontic tooth movement. Bone Joint Res 2016;5:492–499. DOI: 10.1302/2046-3758.510.BJR-2016-0004.R2.

Air source absorption heat pump is promising in energy saving and emission reduction of heating and domestic hot water, but performs badly or even cannot work in cold climate. The ammonia/absorbent air source absorption heat pump with low-pressure boosting is proposed to solve the problem. The hybrid air source absorption heat pump + compressor system is modeled and the compression ratio is optimized to obtain maximum primary energy efficiency. The integrated system is simulated with air temperature ranging from −30℃ to 10℃ and hot water temperature from 30℃ to 60℃. Comparative simulations on three working fluids reveal that NH3–LiNO3 system has the lowest compression ratio and the highest primary energy efficiency value. By pressure boosting, the air source absorption heat pump can operate under air temperatures as low as −30℃. Primary energy efficiency of the hybrid system is about 15–50% higher than that of coal boiler, showing great potential for heat supply in cold regions. Practical application: Heating and domestic hot water consumes a large amount of energy every year. Air source absorption heat pump can be a potential alternative to the traditional boiler systems in the point view of primary energy efficiency. However, air source absorption heat pump performs badly or cannot work when the air temperature is low. This paper presents a hybrid air source absorption heat pump with pressure boosting to improve the performance of the air source absorption heat pump heating system, making it operate under lower outdoor air temperatures with higher primary energy efficiency. The novel heat supply system is expected to make contributions to building energy saving as well as pollution reduction.

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