Background:Triple-negative breast cancer (TNBC) accounts for 15–20% of all breast cancer in women globally. This subtype often has early and high recurrence rates, resulting in poor survival, partially due to lack of targeted therapies. To date, the detailed molecular mechanisms underlying TNBC progression are unclear. Given the crucial role of microRNAs (miRNAs) in cancer metastasis, we aimed to analyse the expression and function of a metastasis-associated miRNA named miR-211-5p in TNBC.Methods:MiRNA array analysis was performed to search for metastasis-associated miRNAs in TNBC. The miR-211-5p expression in tumour tissues, adjacent non-tumourous breast tissues of TNBC patients and cell lines were evaluated by real-time PCR. The protein expression levels were analysed by western blot, immunohistochemistry and in situ hybridisation. Luciferase reporter assays were employed to validate the target of miR-211-5p. The effect of miR-211-5p on TNBC progression was investigated in vitro and in vivo.Results:MiR-211-5p was significantly downregulated in TNBC, and its expression level was associated with overall survival in TNBC. The expression of miR-211-5p suppressed TNBC cell proliferation, invasion, migration and metastasis in vitro and in vivo. Furthermore, SETBP1 was identified as a target of miR-211-5p. Through gain-of-function and loss-of-function studies, SETBP1 was shown to significantly affect colony and cell number in vitro. Enforced expression of miR-211-5p inhibited the expression of SETBP1 significantly and the restoration of SETBP1 expression reversed the inhibitory effects of miR-211-5p on TNBC cell proliferation and metastasis.Conclusions:These findings collectively demonstrate a tumour suppressor role of miR-211-5p in TNBC progression by targeting SETBP1, suggesting that miR-211-5p could serve as a potential prognostic biomarker and therapeutic target for TNBC.

Background: The aim of this study was to compare the complication rates between completion thyroidectomy and primary total thyroidectomy for differentiated thyroid cancer (DTC). Methods: PubMed, the Web of Knowledge, and the China Journal Net were searched for studies concerning the treatment of DTC published in 1990-2014. A meta-analysis was performed to compare the effects of different treatments. Results: 7 studies with a total of 1,208 patients were included. There were no statistically significant differences regarding the presence of temporary recurrent laryngeal nerve (RLN) palsy, permanent RLN palsy, temporary hypocalcemia, permanent hypocalcemia, hematoma, and wound infection. Conclusions: Completion thyroidectomy can be performed with acceptable morbidity in select cases of DTC who could not be properly diagnosed perioperatively or who recurred after less than total thyroidectomy.

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This study is concerned with the unified analytical solution of rotor strength for the segmented permanent magnet (PM) rotor retained by the carbon-fibre sleeve and non-magnetic alloy sleeve in high-speed surface-mounted permanent magnet synchronous machine. Considering the influence of different densities and coefficients of thermal expansion of PMs and pole fillers on rotor stress, the analytical solution for rotor strength was proposed based on displacement method and stress potential method in polar coordinate. The proposed analytical solution was validated by the finite-element method (FEM) and experiment, respectively, and the influences of rotational speed, retaining sleeve thickness and interference fit between the retaining sleeve and PMs on rotor strength were further investigated based on the analytical solution proposed. It is shown that the results calculated by the proposed analytical solution and the FEM are in good agreement with each other, and the analytical solution can accurately predict the stress distributions of the segmented PM rotor retained by the carbon-fibre sleeve and non-magnetic alloy sleeve. The difference in density and coefficient of thermal expansion between PM and pole filler, rotational speed and operating temperature have great effects on rotor strength.

Fungi residing in mangroves are considered to be a bank of novel bioactive natural products. In the screening for bioactive metabolites from mangrove-derived fungi, the ethyl acetate extract of the fermentation broth of Aspergillus fumigatus JRJ111048, a fungus isolated from the leaves of the mangrove plant Acrostichum specioum endemic to Hainan island, was found to possess insecticidal activity against Spodoptera litura. Bioactivity-guided isolation lead to the discovery of seven metabolites 1–7, including one new anhydride derivative aspergide (1), one new lipid amide 11-methyl-11-hydroxyldodecanoic acid amide (2), and five known compounds; α-ethyl glucoside (3), spiculisporic acid B (4), spiculisporic acid C (5), spiculisporic acid (6), and secospiculisporic acid B (7). Their structures were established by NMR spectroscopic and MS analyses, and by comparison of previously reported data. Insecticidal activity against S. litura and antifungal activity of these compounds were investigated. As a result, the new compound 1 showed potent insecticidal activity against newly hatched larvae of S. litura, and compound 4 displayed weak antifungal activity against Candida albicans.