Simultaneous detection of large viruses has been a great obstacle in the field of molecular imprinting. In this work, for the first time, a multifunctional molecularly imprinted sensor for single or simultaneous determination of hepatitis A virus (HAV) and hepatitis B virus (HBV) is provided. Visual detection was realized due to the color of green and red quantum dots that varied with the concentration of the target substance. The combination of hydrophilic monomers and metal chelation reduced the nonspecific binding and enhanced the specificity of adsorption. As a result, satisfactory selectivity and sensitivity were obtained for the detection of the two viruses, with imprinting factors of 3.70 and 3.35 for HAV and HBV, and limits of detection of 3.4 and 5.3 pmol/L, respectively, that were achieved within 20 min. The excellent recoveries during simultaneous detection and single detection modes indicate the prominent ability of the proposed sensor to detect HAV and HBV in human serum and the potential ability to simultaneously detect multiple viruses in real applications.

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A magnetic resonance light scattering (RLS) sensor based on the molecularly imprinted polymer (MIP) technique was developed for specific recognition of trace quantities of hepatitis A virus (HAV).

Protein glycosylation plays a critical role in post-translational modifications of proteins in the organism and is involved in many diseases. However, the huge challenge for glycoproteins to be highly specific isolated and adsorbed from complicated biological samples results from their low abundance and interference. In this work, a novel dual-functionalized magnetic metal-organic frameworks nanoparticle for selective enrichment of glycoproteins was synthesized for the first time. Due to the abundant amino groups and grafted phenylboronic acid, the proposed nanoparticles have the dual properties of hydrophilicity and boronic acid affinity. The obtained nanoparticles show high binding capacities toward glycoproteins under physiological state (pH 7.4) such as ovalbumin (327.28 mg/g), transferrin (241.17 mg/g), horseradish peroxidase (530.79 mg/g). Furthermore, the nanoparticles still have excellent enrichment performance after being used six times repeatedly. More importantly, the prepared nanoparticles also have great potential applications in the adsorption of glycoproteins from complex biological specimens.