Metal-coordination-directed biomolecule crosslinking in nature has been used for synthesizing various biopolymers,including DNA, peptides,proteins,and polysaccharides. However,the RNAbiopolymer has been avoided so far,asdue to the poor stability of the RNAm olecules,t he formation of ab iopolymer may alter the biological function of the molecules.H erein, for the first time,w er eport Zn 2+ -driven RNAs elf-assembly forming spherical nanoparticles while retaining the integrity and biological function of RNA. Various functional RNAs of different compositions,shapes,and lengths from 20 to nearly 1000 nucleotides were used, highlighting the versatility of this approach. The assembled nanospheres possess as uperior RNA-loading efficiency,p harmacokinetics, and bioavailability.I n-vitro and in-vivo evaluation demonstrated mRNAd elivery for expressing GFP proteins,a nd microRNAd elivery to triple-negative breast cancer.T his coordination-directed self-assembly behavior amplifies the horizons of RNAc oordination chemistry and the application scope of RNA-based therapeutics.

Nano-antibacterial agents play a critical role in chronic wound management. However, an intelligent nanosystem that can provide both visual warning of infection and precise sterilization remains a hurdle. Herein, a...

Metal-coordination-directed biomolecule crosslinking in nature has been used for synthesizing various biopolymers,including DNA, peptides,proteins,and polysaccharides. However,the RNAbiopolymer has been avoided so far,asdue to the poor stability of the RNAm olecules,t he formation of ab iopolymer may alter the biological function of the molecules.H erein, for the first time,w er eport Zn 2+ -driven RNAs elf-assembly forming spherical nanoparticles while retaining the integrity and biological function of RNA. Various functional RNAs of different compositions,shapes,and lengths from 20 to nearly 1000 nucleotides were used, highlighting the versatility of this approach. The assembled nanospheres possess as uperior RNA-loading efficiency,p harmacokinetics, and bioavailability.I n-vitro and in-vivo evaluation demonstrated mRNAd elivery for expressing GFP proteins,a nd microRNAd elivery to triple-negative breast cancer.T his coordination-directed self-assembly behavior amplifies the horizons of RNAc oordination chemistry and the application scope of RNA-based therapeutics.