Sarcocystis hominis, one of the three species of Sarcocystis that cause muscular cysts in cattle, is a protozoan parasite that can infect the human intestinal tract. The objective of the present study was to develop a new molecular identification method capable of discriminating among the bovine Sarcocystis species and to apply this tool in combination with stereomicroscopy to determine the presence of Sarcocystis spp. in minced beef in Belgium, with special attention to Sarcocystis hominis. A PCR technique based on the 18S rRNA sequence and by sequencing of the amplicon was highly specific. Sequence analysis of PCR products from thick-walled cysts collected from minced beef in Belgium revealed that S. hominis was present in 97.4% of the samples. Because the consumption of raw minced beef is common in Belgium and certain other European countries, these findings may point to an underestimated risk to public health.
Tijdens de post-mortemkeuring en het versnijden van een rund worden grijsgroene ovale spierletsels vaak als sarcosporidiosis bestempeld, terwijl de morfologische diagnose boviene eosinofiele myositis is. Deze verwarring in terminologie kan niet alleen financiële implicaties hebben, maar leidt ook tot het invoeren van incorrecte gegevens in Europese databanken. Dit artikel vat de huidige kennis samen omtrent Sarcocystis en boviene eosinofiele myositis bij het rund, als pleidooi voor een correct gebruik van de terminologie.
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