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Isoquinoline alkaloids are the primary active ingredients of Corydalis, but an analytical method for quality assessment of the active ingredients in Corydalis impatiens (Pall). Fisch has not been reported. A new, simple, and multiple-component quantification method was developed for the simultaneous quantification of 11 isoquinoline alkaloids including capnoidine, chelianthifoline, bicuculline, protopine, isoapocavidine, apocavidine, cavidine, tetrahydroepiberberine, ochotensimine, tetrahydrocoptisine, and tetrahydrocorysamine in C. impatiens. Separation of the isoquinoline alkaloids was performed on a RP C18 column (150 × 4.6 mm, 5 μm) with potassium dihydrogen phosphate buffer (pH 2.5, adjusted by phosphoric acid)/acetonitrile (53:47, v/v) containing 0.3% sodium dodecyl sulfonate. The flow rate and detection wavelength were set at 1 mL/min and 295 nm, respectively. Full validation of the assay was carried out including linearity, precision, accuracy, stability, LOD, and limit of quantitation. All calibration curves showed a good linear relationship (r > 0.999) in test range. The results demonstrated that the developed method was reliable, rapid, and specific. Six batches of C. impatiens samples from different sources were determined using the established method. The contents of alkaloids ranged from 11.68 to 351.83 μg/g. This method can be applied for quality evaluation and control of C. impatiens. Eleven isoquinoline alkaloids were first reported on simultaneous determination with HPLC.

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In this study, 102 patients with hypertensive intracerebral hemorrhage (52 patients in the citicoline treatment group and 50 patients in the routine treatment group) were analyzed and compared. It was found that citicoline-assisted treatment of hypertensive intracerebral hemorrhage could inhibit the release of serum glial fibrillary acidic protein (GFAP) and peptides ( P < 0.05) and improve the prognosis and neurological and daily living ability of patients ( P < 0.05). In addition, CDP-choline reduced the mortality of neurons, inhibited inflammatory factors, and improved the prognosis of patients ( P < 0.05). A high dose of CDP-choline was also significantly effective in protecting neurons.