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In this paper, extracts from male zooid of Antheraea pernyi (EMZAP) were investigated for their antiobesity activity in C57BL/6 male mice. Mice were randomly divided into 4 groups and fed on basal control group diet (Control Group), high-fat diet (Model Group), high-fat diet containing high dose of EMZAP (High Group), and high-fat diet containing low dose of EMZAP (Low Group), respectively. The levels of serum lipid, antioxidant capacity, body weight, and adipose weight were measured. The levels of expression of genes involved in cholesterol metabolism were also determined by Real-Time PCR. The results showed that EMZAP could reduce body weight and epididymal adipose weight of mice on fed high-fat diet, and also decrease serum lipid levels in mice with induced obesity. Treatment with EMZAP also produced significant amelioration of lipid accumulation and improved the antioxidant capacity in the liver. EMZAP reduced the expression of HMG-CoA reductase (HMG-CoA-R) and increased the expression of cholesterol 7-alpha hydroxylase (CYP7A1) and LDL receptor (LDLR). But it has no obvious effect on the expression of LXRα. According to the above results, EMZAP had good anti-obesity attributes and improvement in conditions arising out of cholesterol accumulation.

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Background: Inflammatory demyelination and axonal injury of the optic nerve are hallmarks of optic neuritis (ON), which often occurs in multiple sclerosis and is a major cause of blindness in young adults. Although a high dose of corticosteroids can promote visual recovery, it cannot prevent permanent neuronal damage. Novel and effective therapies are thus required. Given the recently defined capacity of matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, in immunomodulation and neuroprotection, we tested in this study the effect of matrine on ON in rats with experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Results: MAT administration, started at disease onset, significantly suppressed optic nerve infiltration and demyelination, with reduced numbers of Iba1+ macrophages/microglia and CD4+ T cells, compared to those from vehicle-treated rats. Increased expression of neurofilaments, an axon marker, and decreased apoptosis in retinal ganglion cells (RGCs) were also observed after MAT treatment. Conclusions: Taken as a whole, our results demonstrate that MAT attenuated inflammation, demyelination and axonal loss in the optic nerve, and protected RGCs from inflammation-induced cell death. MAT may therefore have potential as a novel treatment for this disease that causes blindness.