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Abstracts: This article, by combining field investigation with laboratorial analysis, studies diverse alpine meadow at the Eastern Margin of the Qinghai-Tibet Plateau for the underground biomass dynamics, vertical distribution of the content of soil carbon and nitrogen, the connection between the biomass and the content of carbon and nitrogen. The studies show that underground biomass in the herb layer of upland meadow is more than that in the terrace meadow, while underground biomass in the upland shrubland is the most. The vertical distribution of underground biomass of each type is obvious as in shape of "T". As to the distribution of the content of soil organic carbon in the three sample grounds, it showed that the deeper the soil the less the content of soil organic carbon. In May, unlike at terrace meadow, the underground biomass and the content of soil organic carbon in positive proportion, such revelation at upland meadow and upland shrubland is not apparent. In July, at upland meadow and terrace meadow the underground biomass and the content of soil total nitrogen in positive proportion, such revelation at upland shrubland is not apparent either.

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Objective To explore the evaluation value of N-terminal pro-brain natriuretic peptide (NT-proBNP) and cytokines in predicting severe and therapeutic retinopathy of prematurity (ROP).MethodsPremature infants from January 2020 to January 2022 were enrolled in this single-center retrospective study. General characteristics, seven cytokines within 24 hours and NT-proBNP on the 1st, 3rd, 7th and 14th days after birth were collected. Evaluated these risk factors by using univariate Logistic regression and multivariable logistic regression. Receiver operating characteristic curve analysis was used to evaluate the prediction ability. The outcome measures were the occurrence of any stage ROP, severe ROP and therapeutic ROP.Results528 premature infants were involved, 21.4% premature infants (113/528) were diagnosed with ROP, 35.4% (40/113) were diagnosed with severe ROP, 65% (26/40) received intravitreal anti-vascular endothelial growth factor treatment. After adjusting confounding factors, NT-proBNP at day 7 of life (NT-proBNP7) and IL-6 still remained significantly associated with ROP (P < 0.05). For severe ROP, the cut off values for IL-6 and NT-ProBNP7 were respectively 16.28 pg/mL (AUC = 0.830) and 2553ng/L (AUC = 0.846). The AUC value for combining the two indices was 0.919. For therapeutic ROP, the cut off values for IL-6 and NT-ProBNP7 were respectively 18.08 pg/mL (AUC = 0.845) and 4699ng/L (AUC = 0.829). The AUC value for combining the two indices was 0.915.ConclusionsNT-proBNP 7 and IL-6 can be used as early biomarkers for severe and therapeutic ROP. More importantly, two indicators have significantly higher efficacy than individual indicators.