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After long periods of storage, plant pathogen isolates lose their sporulation capacity. The objective of this study was to evaluate re-isolation methodologies for recovering sporulation of Pestalotiopsis grandis-urophylla isolates after subjection to a long period of storage. Isolates of P. grandis-urophylla were kept for 14 months on Petri dishes with PDA medium at 10°C. After this period, the isolate colonies showed reduced mycelial growth and no sporulation. The isolates were inoculated on healthy Eucalyptus grandis-urophylla leaves, and after ten days they were subjected to three re‑isolation methods: scraping of the lesions (S) removing of injured plant tissue fragments, followed by disinfestation (D) and without disinfestation (WD). Then, the purified isolates were evaluated for the recovery of its sporulation ability. The different methods for re-isolation resulted in the occurrence of differences among the isolates, showing that sporulation is an isolate-dependent feature. The three methods (S, WD and D) allowed the sporulation recovery of P. grandis-urophylla, even after these isolates have been subjected to 14 months.

<p>Objetivou-se avaliar diferentes doses de ácidos húmicos na obtenção de massa fresca inicial de plantas de feijão comum ‘Pérola’. Para tanto, sementes de feijão foram tratadas com Rhal S1^® (18% de ácidos húmico + 1,5% de ácidos fúlvicos) nas doses de 0, 100, 200, 300, 400 e 500 mL da solução para cada 100 kg de sementes. Em seguida, as sementes foram semeadas em vasos de 500 g de capacidade e após 14 dias de cultivo em casa de vegetação, foram mensurados a massa fresca da parte aérea e massa fresca das raízes. O experimento foi disposto em delineamento inteiramente casualizados (DIC)com quatro repetições (1 planta por vaso; 4 plantas por tratamento)e os resultados foram submetidos, à análise de regressão. As equações de regressão obtiveram resposta quadrática para todas a variáveis analisadas em função das doses aplicadas. As plantas de feijão responderam pelo aumento de massa fresca da parte aérea até a dose de 200 mL de Rhal S1^® 100 Kg^-1 sementes, enquanto que amassa fresca das raízes respondeu até a dose de 100 mL de Rhal S1^® 100 Kg^-1 sementes, confirmando maior demanda da parte aérea por ácidos húmicos do que as raízes.</p><p align="center"><strong><em>Humic acids to </em></strong><strong><em>obtain higher early fresh matter in ‘Pérola’ common bean plants</em></strong></p><p><strong>Abstract: </strong>It is aimed to evaluate different doses of humic acidto obtain early fresh matter of ‘Pérola’ common bean plants. Therefore, common bean seeds were treated with Rhal S1^® (18% of humic acid + 1.5% of fulvic acids) at doses of 0, 100, 200, 300, 400 and 500 mL of solution for each 100 kg seeds. Just after, the seeds were sown in 500 g capacity pots and after 14-days cultivation in greenhouse, it was measured the fresh matter of the aerial part and fresh matter of the roots. The experiment was carried out in completely randomized design (CRD) with four replicates (1 plant per pot; 4 plants per treatment) and the results of the two experiments were subjected, together, to regression analysis. The regression equations obtained quadratic response for all variables evaluated in function of the applied doses. The common bean plants respond by the increasing of fresh matter of aerial part up to 200 mL of Rhal S1^® 100 Kg^-1 seeds, while the fresh matter ot the roots increasing up to 100 mLof Rhal S1^® 100 Kg^-1 seeds, confirming greater demand of shoots by humic acids than the roots.</p>

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