Aboveground biomass (AGB) is an important indicator used to predict crop yield. Traditional spectral features or image textures have been proposed to estimate the AGB of crops, but they perform poorly at high biomass levels. This study thus evaluated the ability of spectral features, image textures, and their combinations to estimate winter wheat AGB. Spectral features were obtained from the wheat canopy reflectance spectra at 400–1000 nm, including original wavelengths and seven vegetation indices. Effective wavelengths (EWs) were screened through use of the successive projection algorithm, and the optimal vegetation index was selected by correlation analysis. Image texture features, including texture features and the normalized difference texture index, were extracted using gray level co-occurrence matrices. Effective variables, including the optimal texture subset (OTEXS) and optimal normalized difference texture index subset (ONDTIS), were selected by the ranking of feature importance using the random forest (RF) algorithm. Linear regression (LR), partial least squares regression (PLS), and RF were established to evaluate the relationship between each calculated feature and AGB. Results demonstrate that the ONDTIS with PLS based on the validation datasets exhibited better performance in estimating AGB for the post-seedling stage (R2 = 0.75, RMSE = 0.04). Moreover, the combinations of the OTEXS and EWs exhibited the highest prediction accuracy for the seeding stage when based on the PLS model (R2 = 0.94, RMSE = 0.01), the post-seedling stage when based on the LR model (R2 = 0.78, RMSE = 0.05), and for all stages when based on the RF model (R2 = 0.87, RMSE = 0.05). Hence, the combined use of spectral and image textures can effectively improve the accuracy of AGB estimation, especially at the post-seedling stage.

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