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Supramolecular encapsulation, which removes harmful substances from organisms, has evolved into a new strategy. In this paper, three supramolecular complexes of acyclic cucurbit[n]urils (ACBs) with uric acid (UA) were prepared, and the inclusion behavior of ACBs and UA was studied by fluorescence spectroscopy, UV–vis spectroscopy and nuclear magnetic resonance. Furthermore, the effect of the complexes of UA with ACBs on the expression of inflammatory biomarkers in human hepatoma HepG2 cell lines was characterized through C-reactive protein (CRP) western blot. The results showed UA molecules can be recognized by three ACBs with different binding constants, and ACBs successfully blocked the inflammatory stimulation of UA on HepG2 cell lines and inhibited the expression of the major inflammatory factor CRP by the formation of complexes between UA and ACBs. This article proves that ACBs can efficiently reverse the cytotoxicity of UA, which provides a new method for treating hyperuricemia disease.

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Genistein (Gen) has great potential in the field of anti-tumor, however, its low solubility in water, serious biotoxicity on thyroid function and estrogenic effects limit its further clinical application. Host-guest inclusion complex based on supramolecular chemistry could be a promising strategy to address these issues. During last decades, cyclodextrin as one of the most popular supramolecular hosts have been considered as a promising drug delivery carrier. Herein, the host-guest encapsulation system of Gen with amino-appended β-cyclodextrins (ACDs) were reported, and inclusion complexes have been characterized by 1 H and 2D-ROESY nuclear magnetic resonance (NMR), Phase solubility curve, Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The results showed that Gen was successfully encapsulated into the cavities of ACDs to form the complexes with 1 : 1 stoichiometry, and compared with free Gen, the aqueous solubility of the complexes was enhanced by 1000 folds. In addition, the cytotoxicity of their complexes to tumor cell line HEK293T was significantly improved. These results will be potentially useful for the development of further clinical application of genisteinbased anticancer agent.