No abstract

BackgroundRat renal transplantation is an essential experimental model for studies of transplantation immunobiology. Harvesting both kidneys from one donor rat for transplantation is widely used to reduce the number of experimental animals. Using the conventional method, both kidneys of the donor rat are harvested simultaneously, which leads to the prolonged warm ischemic times during transplantation of the second donor kidney. Prolonged warm ischemia time is the main risk factor for delayed graft function.MethodsTwo different approaches are compared. Method 1, conventional method: both kidneys of the donor rat are harvested simultaneously and then transplanted into two recipients. During transplantation, the first and second donor kidneys were regarded as Group 1 and 2, respectively. Method 2, step-by-step method: after left nephrectomy, the donor rat survives, and we perform left renal transplantation (Group 3). Then, the right kidney of the surviving donor rat is incised and transplanted into the left side of the second recipient (Group 4).ResultsThe success rates were 86.7, 93.3, 93.3 and 86.7% in groups 1, 2, 3 and 4, respectively. The warm ischemia times increased significantly in group 2 compared with the other 3 groups (p < 0.05) but differed non-significantly between groups 3 and 4 (p > 0.05). Serum creatinine levels, blood urea nitrogen and 24-h urine protein level obviously increased after kidney transplantation in group 2 compared with other groups (p < 0.05).ConclusionsWe developed an optimized method for reducing warm ischemia time, thereby minimizing delayed graft function.

Man, 55-year-old, developed Sagliker syndrome (SS) in the course of hemodialysis for end-stage renal disease from 2008, as a result of uncontrolled secondary hyperparathyroidism (SHPT). His medical managements failed and he had no chance of kidney transplantation. Although surgical parathyroidectomy was rejected and the disease development could not be reversed, PTH began to decline and SS progressed slowly under the control of management and guidance in medication treatment. It is possible that such patients can survive with long-time dialysis, but the prevention of severe SHPT is critical.