Through years, the reported intracellular H 2 O 2 sensors just focused on the unrelated measurements of the intracellular H 2 O 2 generated from the stimulus of Cd 2+ , ascorbic acid (AA) etc., leading to difficulty in data interpretation. Here, a novel reduced graphene oxide quantum dots(rGO QDs)/ZnO hybrid nanofibers-based electrochemical biosensor for the detection of intracellular H 2 O 2 released from cancer and normal cells under the stimuli of the corresponding anticancer drugs permits a quantitative study of the interaction between the target drug compound and the cancer cell, which is suitable for candidate drug screening. Nylon 6/6 nanofibers are used as robust templates for the facile fabrication of novel rGO QDs/ZnO hybrid nanofibers via an electrospinning followed by a step hydrothermal growth method. The as-made sensor was applied to determine the H 2 O 2 released from prostate cancer cell (PC-3) versus noncancerous cell (BPH-1) under the stimuli of the corresponding anticancer drugs (apigenin, antisense CK2α etc). The amount of the H 2 O 2 released from the PC-3 cancer cell is about (320±12) amol cell -1 and about (210±6) amol cell -1 for the BPH-1 noncancerous cell under the stimuli of specific therapy drug antisense CK2α. These results demonstrate that rGO QDs/ZnO hybrid nanofibers-based electrochemical biosensor can efficiently detect the distinct amounts of H 2 O 2 released from cancer and noncancer cells.

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A peculiar tower-like ZnO nanostructure was synthesized using an economical and facile hydrothermal method, with zinc acetate [ZAc, Zn(CH3COO)2 · 2H2O] and hexamethylenetetramine (HMTA, C6H12N4) as the source materials. The as-synthesized tower-like ZnO was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The characterization results indicated that the tower-like ZnO nanostructures are high-quality monocrystals. The as-synthesized ZnO nanostructures may have application in sensing area due to its high specific surface areas. The growth mechanism, as well as the optical properties of the as-synthesized tower-like ZnO nanostructures was also studied.

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