Chrysanthemum morifolium (Asteraceae family) have long been used as a tonic, antioxidant, antipyretic, analgesic, sedative, antitumor, neuroprotector, hepatoprotector and cardioprotector agent. This species should be reconsidered as possible sources of many biocompounds, especially amino acids. Thus, the aim of this study was to validate the chromatographic method for detection of amino acids and their identification in flowers and leaves of Ch. morifolium of variant Pectoral. HPLC-FLD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The calibration curves of all analytical standards of amino acids were linear (R2 > 0.99) over the range of 0.015–0.625 μmol/mL, the LODs and the LOQs were in the range of 0.001–0.096 µg/mL and 0.004–0.321 µg/mL, respectively. During the HPLC-FLD assay ten amino acids in free form and fifteen amino acids after hydrolysis in Ch. morifolium flowers were identified. Besides, twelve amino acids were detected in free form and fourteen amino acids after hydrolysis in Ch. morifolium leaves. The results of HPLC-FLD analysis showed that the predominant amino acid was L-proline in both types of herbal raw materials. Its total content was 31.67±0.02 μg/mg in Ch. morifolium flowers and 18.56±0.02 μg/mg in Ch. morifolium leaves. This phytochemical study confirms that flowers and leaves of Ch. morifolium (Pectoral) are rich sources of amino acids and can exhibit a wide range of pharmacological activities.

Medicinal plants and their combinations can influence various links of the pathogenetic mechanism of diabetes mellitus type 2 and its complications, due to the wide range of biologically active substance that they accumulate. Flavonoids deserve particular attention through their antioxidant properties. Three samples of herbal mixtures (sample 1 – Inula helenium rhizome with roots, Helichrysum arenarium flowers, Zea mays columns with stigmas, Origanum vulgare herb, Rosa majalis fruits, Taraxacum officinale roots; sample 2 – Cichorium intybus roots, Elymus repens rhizome, Helichrysum arenarium flowers, Rosa smajalis fruits, Zea mays columns with stigmas; sample 3 – Urtica dioica leaf, Taraxacum officinale roots, Vaccinium myrtillus leaf, Rosa majalis fruits, Mentha x Menthapiperita herb) were tested for flavonoid content and antioxidant properties. Using HPLC-DAD analysis the content of flavonoids was evaluated and an antioxidant activity by DPPH-radicals scavenging, ferrous ion chelating capacity and ferric reducing power were established for the herbal mixtures. Rutin prevails in sample 3, its content was 2745.66±0.21 μg/g; luteolin – in samples 1 and 2, its content was 371.31±0.07 μg/g and 313.48±0.13 μg/g, respectively. Flavonoids attribute to the antioxidant activity of the herbal mixtures, which was confirmed by DPPH radical scavenging assay, ferric reducing power assay and ferrous ion chelating assay. The highest antioxidant capacity was found for sample 3 – IC50 of inhibition of DPPH radicals was 301.65±2.67 µg/mL compared to control – ascorbic acid (119.24±2.35 µg/mL), the ferric reducing power was 0.382 at 100 µg/mL compared to ascorbic acid (0.412 at 100 µg/mL) and IC50 of chelating capacity was 206.59±2.48 µg/mL compared to EDTA-Na2 (110.55±1.93 µg/mL).