HPLC-FLD analysis of amino acids content in Chrysanthemum morifolium

Abstract

Chrysanthemum morifolium (Asteraceae family) have long been used as a tonic, antioxidant, antipyretic, analgesic, sedative, antitumor, neuroprotector, hepatoprotector and cardioprotector agent. This species should be reconsidered as possible sources of many biocompounds, especially amino acids. Thus, the aim of this study was to validate the chromatographic method for detection of amino acids and their identification in flowers and leaves of Ch. morifolium of variant Pectoral. HPLC-FLD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The calibration curves of all analytical standards of amino acids were linear (R2 > 0.99) over the range of 0.015–0.625 μmol/mL, the LODs and the LOQs were in the range of 0.001–0.096 µg/mL and 0.004–0.321 µg/mL, respectively. During the HPLC-FLD assay ten amino acids in free form and fifteen amino acids after hydrolysis in Ch. morifolium flowers were identified. Besides, twelve amino acids were detected in free form and fourteen amino acids after hydrolysis in Ch. morifolium leaves. The results of HPLC-FLD analysis showed that the predominant amino acid was L-proline in both types of herbal raw materials. Its total content was 31.67±0.02 μg/mg in Ch. morifolium flowers and 18.56±0.02 μg/mg in Ch. morifolium leaves. This phytochemical study confirms that flowers and leaves of Ch. morifolium (Pectoral) are rich sources of amino acids and can exhibit a wide range of pharmacological activities.

Amino acid HPLC-FLD analysis of spirulina and its protective mechanism against the combination of obesity and colitis in wistar rats

“…Spirulina sample was then maintained for 24 h in the oven at 105 °C to facilitate the hydrolysis of AA chains, including peptides and protein into individual amino acids. Eventually, 6 ml NaOH (6 N) was added to 2 ml of the hydrolyzed HCl sample [ 28 ]. The resulting extracts underwent centrifugation at 3500 rpm and 1 ml of supernatants were evaporated using a rotary evaporator, with 3 washes performed using distilled water to remove residual HCl [ 29 ].…”

Phytochemicals, therapeutic benefits and applications of chrysanthemum flower: A review

“…Using HPLC-FLD assay, a total of fifteen amino acids after hydrolysis and ten amino acids in free form were identified in C. morifolium flowers, involving l -aspartic acid, l -serine, l -glutamic acid, l -histidine glycine, l -threonine, l -arginine, l -alanine, l -tyrosine, l -valine, l -phenylalanine l -isoleucine, l -lysine l -leucine and l -proline. Besides, other essential amino acids, l -lysine was found in higher amount 12.05 ± 0.02 μg/mg [ 25 ]. A recent study found that C. morifolium flower extract (CME) shows 1.96 nmol/mg protein in 0.2% CME samples [ 30 ].…”

A novel bio-electrochemical sensor based on a 1,4-bis(triphenylphosphonium)butane)3[SiW11O39Ni(H2O)]/P@ERGO nanocomposite for the selective determination of l-cysteine and l-tryptophan

“…11,12 Therefore, accurate, simple, and fast methods are desired for determining Cys and Trp in many fields, such as food processing, biotechnology, and pharmaceutical industries. [13][14][15][16] Numerous analytical methods have been developed for amino acid quantification, e.g., high-performance liquid chromatography (HPLC), 17,18 spectrophotometry, 19 gas chromatography-mass spectroscopy (GC-MS), 20 and electrochemistry. 1,4,16 Among these methods, electrochemical procedures have attracted considerable interest in electro-active compounds because of their simplicity, low-cost, high sensitivity, and ease of miniaturization.…”