The use of xenotissue for the needs of regenerative and cardiac medicine is a promising area of tissue engineering. The decellularization process provides complete purification of the elastin-collagen matrix of the bovine pericardium from cells and their components. The use of high concentrations of sodium dodecyl sulfate and glutaraldehyde can lead to a damage of the matrix architectonics. Therefore, the purpose of this study was to study the microarchitectonics of the decellularized matrix using a low concentration of sodium dodecyl sulfate (0.1% solution) and avoiding glutaraldehyde. Further stabilization and fixation of the matrix was carried out using 10 mM 1-Ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride and 10 mM N-Hydroxysuccinimide. The effect of decellularization was assessed by staining the samples with hematoxylin-eosin and by scanning electron microscopy. The research results confirmed the absence of structural changes in the collagen-elastin fibers of the matrix. Thus, the efficiency of using the proposed technology for obtaining a decellularized matrix based on the bovine pericardium was confirmed.
The use of xenotissue for the needs of regenerative and cardiovasculare medicine is a promising area of tissue engineering. The decellularization process provides complete purification of the elastin-collagen matrix of the bovine pericardium from cells and their components. The use of high concentrations of sodium dodecyl sulfate and glutaraldehyde can lead to a damage of the extracellular matrix. Therefore, the purpose of this study was to study the microarchitectonics of the decellularized matrix using a low concentration of sodium dodecyl sulfate (0.1% solution) and avoiding glutaraldehyde. Further stabilization and fixation of the matrix was carried out using 10 mM 1- carbodiimide hydrochloride and 10 mM N-Hydroxysuccinimide. The effect of decellularization was assessed by staining the samples with hematoxylin-eosin and by scanning electron microscopy. Also the research results confirmed the absence of structural changes in the collagenelastin fibers of the matrix after sterilization dose of 10 kGy. Thus, it can be assumed that the radiation method of sterilization may be safe in use for sterilization of bioimplants.
The rapid growth of cardiovascular morbidity and high mortality rates of patients with congenital heart disease requiring surgery have led to the search for new modern approaches to the treatment of these groups of patients. The main trends today include the use of cardiaс implants of synthetic and biological origin. Of particular interest are scaffolds based on the decellularized extracellular matrix, which in its functional and structural characteristics is close to the native pericardium. In contrast to synthetic analogues, such grafts can fully replace a tissue or an organ defects, and then integrate and function properly. This review presents the characteristics of different types of matrices used in cardiac surgery. The advantages and disadvantages of commercially available cardiac bioimplants currently used in the world are analyzed.
The aim: To investigate the effectiveness of using low concentrations of sodium dodecyl sulfate (SDS) and cross-linking with EDC/NHS in the decellularization process to create a potential bioimplant for cardiac surgery.
Materials and methods: Pericardial sacs were derived from 12-18 months bulls. Tissue decellularization was performed by using 0.1% SDS with the following EDC/NHS cross-linking. The experiment included standard histological, microscopic, molecular genetic and biomechanical methods. Scaffold was tested in vitro for cytotoxicity and biocompatibility.
Results: A high degree of extracellular decellularized matrix purification from cells and their components was shown. Structure-function properties remained similar to those or even improved after the decellularization. During prolonged contact of BP with human fibroblasts, no cytotoxic effect was observed. The biointegration of the scaffold in laboratory animals tissues was noted confirming the potential possibility of the implant use in cardiac surgery.
Conclusions: Decellularization of BP by 0.1 % SDS with NHS/EDC cross-linking is promising in manufacturing of the tissue-engineered materials in cardiac surgery.
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