This study aimed to evaluate sperm chromatin maturity and integrity of that injected into goodquality oocytes in an in vitro fertilization-intra cytoplasmic sperm injection (IVF-ICSI) program. A cut-off value of sperm chromatin maturity and integrity was developed as a function of their correlation to the zygote development, i.e., embryo formation and cleavage rate. The study assessed sperm chromatin maturity using aniline blue (AB) staining, whereas toluidine blue (TB) staining was used to assess sperm chromatin integrity. Ejaculates from 59 patients undergoing ICSI and 46 fertile normozoospermic donors for determination of normal values of sperm chromatin status were used in this study. Embryo formation and cleavage rates were observed for the period of 3 days after ICSI. There was a significant difference in the percentage of sperm with mature chromatin between ejaculate from ICSI patients and fertile donor (p=0.020); while there was no significant difference in sperm chromatin integrity of both samples (p=0.120). There was no significant correlation between sperm chromatin maturity and either embryo formation or cleavage rate; as well as sperm chromatin integrity to both parameters of zygote development (p>0.05). Furthermore, we found that the cut-off value of sperm chromatin maturity and integrity of the fertile normozoospermic ejaculates were 87.2% and 80.2%, respectively. Using the cut-offs, we found that low sperm chromatin maturity at the level of <87% correlated significantly with the cleavage rate of the zygote (p=0.022; r=0.371); whereas poor sperm chromatin integrity at the level of <80% correlated with embryo formation (p=0.048; r=0,485). In conclusion, this study showed that poor maturity and integrity of sperm chromatin (AB<87% and TB<80%, respectively), could affect zygote development following ICSI.

Bengkulu merupakan provinsi yang ke 32 yang terjangkit virus corona Berdasarkan laman Jurnal bengkulu,Tim Gugus Percepatan Penanganan Kasus Covid-19 Provinsi Bengkulu melaporkan pada Juni 2020 total kasus konfirmasi Covid 19 mencapai 101 orang dengan demikian Dinas Kesehatan Provinsi Bengkulu mengumumkan, Bengkulu telah di tetapkan sebagai wilayah transmisi local penularan Covid 19 karena terjadi penularan ke orang pertama dan ke orang-orang di sekitarnya. Pada penelitian ini, penulis bermaksud merancang suatu program aplikasi penentuan krteria pada pasien covid 19 berbasis android. Aplikasi atau system ini menggunakan metode probabilitas bayes dengan Bahasa pemprograman java pada android studio. Berdasarkan percobaan yang telah di lakukan data yang sudah masuk ke dalam sistem yaitu 101 Pasien yang terpapar Covid 19, dengan identifikasi Pasien Dalam Pengawasan (PDP) 25 orang, Orang Dalam Pemantauan (ODP) 35 orang, Kasus Probable 9 orang, Kasus Konfirmasi 6 orang dan Kontak Erat 26 orang.  sistem sudah mampu untuk menentukan kriteria pada pasien covid 19 di provinsi bengkulu dengan rata-rata akurasi system dalam mendiagnosa penyakit berdasarkan gejala klinis dan hasil pengujian dalam berbagai kondisi bernilai sekitar 53,33% dan nilai akurasi tertinggi mencapai 96,66666667. Dengan demikian untu sistem layak digunakan.

Covid-19 merupakan jenis virus baru yang sampai saat ini belum ditemukan obat/antivirus/treatment yang pasti. Penelitian yang dilakukan dan dijelaskan dalam paper ini adalah untuk mengetahui sudut pandang (perspektif) teknologi informasi. Perspective yang dibangun dalam paper ini juga menilik bagaimana teknologi informasi dapat digunakan untuk menangani pandemic-pandemi sebelum Covid-19. Metode penelitian yang digunakan merupakan adaptasi dari penelitian Literatur Review Sistematis yang dilakukan oleh Kitchenham. Dalam paper ini juga akan dibahas tentang strategi intervensi penyebaran Covid-19 diataranya adalah intervensi menggunakan obat (pharmaceutical) dan non-obat (non-pharmaceutical). Pada studi ini juga akan dibahas tentang teknologi informasi yang sudah pernah digunakan di bidang kesehatan sebagai upaya pencegahan penyebaran pandemic-pandemi baik Covid-19 ataupun pandemic lainnya yang pernah terjadi di beberapa negara. Model strategi ini memungkinkan untuk dikembangkan menjadi penelitian selanjutnya untuk dasar penentuan kebijakan strategi berdasarkan penomena yang terjadi yang didasarkan pada data yang di-generate oleh pengguna. Model strategi ini diharapkan bisa dijadikan alternatif strategi untuk menekan biaya belanja pemerintah dan mempersempit ruang gerak transmisi Covid-19

This study aims to determine the effect of administration of Etlingera hemispharica leaf extract on white rats (Rattus norvegicus) induced by mercury chloride as seen from the histopathological description of the testes. The research method used is experimental post-test-only control group design. The extract was administered for seven days by gavage. Testicular organ harvesting was carried out on the 9th day after the rats were euthanized and continued with making testicular preparations. Testicular damage was assessed using the Johnsen score. Data were analyzed using non-parametric statistical tests. The results showed that the test groups had a p-value of 0.000. The histopathological description of the testes induced by HgCl2 had a p-value of 0.005 and the administration of E. hemisphaerica leaf extract in HgCl2-induced mice showed a p-value of 0.008. In conclusion, administration of E. hemisphaerica leaf extract to R. norvegicus induced by mercury chloride improved spermatogenesis on the histopathological features of the R. norvegicus testes. Keywords: Etlingera hemispharica, HgCl2, Johnsen Score, Rattus norvegicus

This study aimed to examine the effect of giving black cumin extract to changes in the histopathological picture of the liver of white rats induced by isoniazid. The method used is experimental with posttest only control group design pattern. The research subjects used 25 Rattus norvegicus, which were divided into negative control group (P1), positive control (P2) isoniazid orally with a dose of 5.4 - 6.75 mg/day for six weeks, treatment 1 (P3), treatment 2 (P4 ), and treatment 3 (P5) which was given isoniazid at a dose of 5.4 - 6.75 mg/day orally and followed by oral black cumin with stratified amounts of 2000, 2500 and 3000 mg/kgBW. The results showed that there was a significant increase in liver cell damage after administration of isoniazid (p < 0.05) and liver cell recovery after administration of black cumin extract for 42 days (p < 0.05). In conclusion, the administration of black cumin extract can restore isoniazid-induced liver cell damage. Keywords: Liver Histopathology, Isoniazid, Black Cumin